Microbial composite organic fertilizer for preventing and controlling wheat scab, and preparation method thereof

A microbial and organic fertilizer technology, which is applied in the field of microbial compound organic fertilizer for the prevention and treatment of wheat scab and its preparation, can solve the problems of human and livestock harm, affecting wheat quality, affecting wheat yield, etc., to increase disease resistance, improve The effect of plant disease resistance and disease resistance improvement

Inactive Publication Date: 2018-10-23
安徽莱姆佳生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Wheat head blight is mainly a disease of wheat crops caused by Gibberella as the main pathogen. It is widely prevalent in the world, and the damage is the most serious in wheat planting areas in temperate regions. In my country, red blight is prone to occur in all wheat planting areas. Mildew, scab not only affects the yield of wheat, but also seriously affects the quality of wheat. At the same time, it will secrete toxins when infecting wheat grains, causing serious harm to humans and livestock. Wheat scab is very easy to spread, and the epidemic area is large, so it is not suitable Control has become a limiting factor for increasing and stabilizing wheat production

Method used

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  • Microbial composite organic fertilizer for preventing and controlling wheat scab, and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0053] A microbial compound organic fertilizer, which is composed of Trichoderma harzianum, Bacillus subtilis, Lactobacillus, and yeast to form a microbial compound agent, plus decomposed straw powder. The specific preparation steps are as follows:

[0054] 1. Preparation of microbial compound bacterial agent:

[0055] Inoculate Trichoderma harzianum on the PDA solid slant medium under aseptic conditions, and cultivate it in an incubator at 30°C for 48 hours. After the green hyphae grow on it, transfer it to PDA liquid medium, and cultivate it at 30°C, 180rpm 72h, then transfer the seed liquid to the fermenter for expansion cultivation, the fermenter volume is 50L, the medium is 30L, the temperature is 30°C, the stirring speed is 180rpm, and the aeration ratio is 0.6vvm. After 72h of fermentation, the fermented liquid Store at low temperature and mix with dried corn flour at a mass ratio of 1:10.

[0056] Inoculate Bacillus subtilis on LB solid medium, culture it in an incuba...

Embodiment 2

[0062] A microbial compound organic fertilizer, which is composed of Trichoderma harzianum, Bacillus subtilis, Lactobacillus, and yeast to form a microbial compound agent, plus decomposed straw powder. The specific preparation steps are as follows:

[0063] 1. Preparation of microbial compound bacterial agent:

[0064] Inoculate Trichoderma harzianum on PDA solid slant medium under aseptic conditions, and culture it in an incubator at 32°C for 46 hours. After the green hyphae grow on it, transfer it to PDA liquid medium, at 32°C, 180rpm Cultivate under conditions for 70 hours, and then transfer the seed liquid to a fermenter for expansion. The volume of the fermenter is 50L, the medium is 30L, and the conditions for expansion: temperature is 32°C, stirring speed is 180rpm, and the aeration ratio is 0.6vvm. After 70 hours of fermentation, the fermented liquid was stored at low temperature and mixed with dried corn flour at a mass ratio of 1:9.

[0065] Inoculate Bacillus subti...

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Abstract

The invention provides a microbial composite organic fertilizer for preventing and controlling wheat scab, and a preparation method thereof. The microbial composite organic fertilizer comprises a microbial composite bacterial agent and an organic waste according to a weight ratio of (4-8):(2-6), wherein the microbial composite bacterial agent comprises Trichoderma harzianum, Bacillus subtilis, Lactobacillus and yeast, and based on the weight of the microbial composite organic fertilizer, the effective viable count of Trichoderma harzianum is not less than 300 million cfu / g, the effective viable cell count of Bacillus subtilis is not less than 500 million cfu / g, the effective viable cell count of Lactobacillus is not less than 300 million cfu / g, and the effective viable cell count of yeastis not less than 300 million cfu / g. According to the present invention, with the microbial composite organic fertilizer, the disease resistance of wheat is significantly improved; and the production process is simple, the strain is easy to culture, the method is suitable for large-scale production, and a certain social and economic benefit can be achieved.

Description

technical field [0001] The invention relates to the technical field of organic fertilizers, in particular to a microbial compound organic fertilizer for preventing and treating wheat scab and a preparation method thereof. Background technique [0002] Wheat head blight is mainly a disease of wheat crops caused by Gibberella as the main pathogen. It is widespread all over the world, and the damage is most serious in wheat planting areas in temperate regions. In my country, red blight is prone to occur in all wheat planting areas. Mildew, scab not only affects the yield of wheat, but also seriously affects the quality of wheat. At the same time, it will secrete toxins when infecting wheat grains, causing serious harm to humans and livestock. Wheat scab is very easy to spread, and the epidemic area is large, so it is not suitable Control has become a limiting factor for increasing and stabilizing wheat production. Contents of the invention [0003] In view of the above-mention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/16C05F11/08C12R1/125C12R1/885C12R1/225C12R1/645
CPCC05F11/08C12N1/14C12N1/16C12N1/20
Inventor 仲松孙善军马忠友汪令翔张印唐飞
Owner 安徽莱姆佳生物科技股份有限公司
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