Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of allicin and derivatives thereof in preparation of medicine for preventing and treating kidney damage caused by urinary calculi and crystals

A technique for urinary calculi and allicin is applied in the field of biomedicine to achieve the effect of good application prospects

Active Publication Date: 2018-10-23
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, surgery is the main treatment for stones, and there are no other medicines for stone-dissolving drugs except potassium hydrogen citrate for cystine stones.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of allicin and derivatives thereof in preparation of medicine for preventing and treating kidney damage caused by urinary calculi and crystals
  • Application of allicin and derivatives thereof in preparation of medicine for preventing and treating kidney damage caused by urinary calculi and crystals
  • Application of allicin and derivatives thereof in preparation of medicine for preventing and treating kidney damage caused by urinary calculi and crystals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 A certain concentration of allicin DATS and DADS has no toxic effect on renal tubular epithelial cells NRK-52E and HK-2

[0042] 1. Materials and methods

[0043] 1. Main reagents

[0044] Dulbecco's Modified Eagle's Medium dry powder (DMEM, low sugar), fetal bovine serum (FBS), trypsin (typsin), dimethyl sulfoxide (dimethyl sulfoxide, DMSO), and Tris were purchased from Sigma-Aldrich (St.Louis, MO, USA); Allicin (Diallyl disulfide, DADS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Allicin (Diallyl trisulfide, DATS) was purchased from Guangdong Drug Control Institute. Other commonly used laboratory reagents are of domestic analytical grade.

[0045]2. Cell culture

[0046] At 37°C, 5% CO 2 And under the condition of saturated humidity, we used DMEM (low glucose) medium containing 10% fetal bovine serum to culture rat renal tubular epithelial cells NRK-52E and human renal tubular epithelial cells HK-2. The cell culture is carried out in a petri...

Embodiment 2

[0068] Example 2 Allicin and its derivatives have inhibitory effect on renal cell damage caused by sodium oxalate

[0069] 1. Experimental method

[0070] The experimental determination method of cell culture, MTT or MTS and the statistical method of other kits are the same as Example 1; 24 hours after the cells are seeded into the 96-well plate, different drug groups are set, and the different concentrations of the drug to be determined are added to the medium. Drug treatment for 24 hours. In the injury experiment caused by sodium oxalate (NaOx), allicin (DATS) was pre-added to the cells for treatment, and after 3 hours, soluble sodium oxalate of different concentrations was added to the cells (sodium oxalate combined with calcium ions in the medium Calcium oxalate crystals were formed later); 24 hours later, the corresponding MTS was added to measure the absorbance of each group, and the corresponding blank control wells were set (because calcium oxalate crystals and drugs ...

Embodiment 3

[0074] Example 3 Allicin and its derivatives have inhibitory effect on crystal adhesion after renal cell injury caused by sodium oxalate

[0075] 1. Experimental method

[0076] Materials are the same as Example 1.

[0077] Crystal adhesion experiment: First, cells were seeded into 6-well plates at a certain density (about 150,000-200,000 cells / well), and after 24 hours, the cells adhered to the wall. Change the medium and add different concentrations of allicin (DATS, DADS) and sodium oxalate (NaOx) to be measured for 24 hours. After aspirating and discarding the culture medium, add the prepared calcium oxalate suspension containing 20ug / cm2 per well, incubate at 37°C for 3 minutes, and then take out the cells. After the cells were washed 5 times with PBS, the cells were placed under a microscope to observe the cells, and the number of adhered crystals on the cell surface was counted under a field of view of 10*4. powerful.

[0078] All experiments were repeated at least ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of allicin and derivatives thereof in the preparation of a medicine for preventing and treating kidney damage caused by urinary calculi and crystals. The first study provided by the invention proves that the allicin has the effects of inhibiting crystal-induced damage and adhesion and reducing the kidney calcium oxalate crystal in a kidney stone model rat, and has the potential to treat urinary calculi. The invention provides a new usage of the allicin and the derivatives thereof, namely the application in preparation of a medicine for preventing and treating kidney damage caused by urinary calculi and crystals, also provides a new selection scheme for the treatment of urinary calculus diseases, and provides diallyl sulfide and derivatives thereof extracted from food such as natural garlic. A research basis is provided for garlic-based urinary calculus diet therapy, and the application prospect is good.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of allicin and its derivatives in the preparation of medicines for preventing and treating renal cell damage caused by urinary stones and crystals. Background technique [0002] Urinary calculus is a common disease worldwide and the most common urological disease in my country. A prerequisite for the formation of stones in urine is the aggregation and nucleation of crystals. The study of the components of urinary calculi found that calcium oxalate is the most common chemical component of urinary calculi. The formation of calcium oxalate stones roughly includes the process of crystal nucleus formation, crystal growth, crystal aggregation, crystal adhesion and retention until stone formation. The existing hypotheses for calcium oxalate stone formation include calcium spot theory, matrix theory, supersaturation and crystallization theory , Inhib...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/255A61K31/10A61K31/095A61P13/12A61P13/04
CPCA61K31/095A61K31/10A61K31/255A61P13/04A61P13/12
Inventor 吴文起赖永长陈东钟芳灵黄健欧莉莉
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com