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A method for determining vitamin D in milk powder

A vitamin and milk powder technology, applied in the field of analytical chemistry, can solve the problems of time-consuming operation and inability to release vitamin D, and achieve the effects of saving labor and cost, simplifying the pretreatment process, and improving sensitivity

Active Publication Date: 2021-03-19
SGS STANDARD TECHNICAL SERVICES SHANGHAI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, repeated ultrasonic extraction and centrifugation is time-consuming; the method of ethanol ultrasonic extraction cannot completely release vitamin D from the complex food matrix

Method used

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  • A method for determining vitamin D in milk powder
  • A method for determining vitamin D in milk powder
  • A method for determining vitamin D in milk powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the mensuration of vitamin D in milk powder

[0038] 1. Sample preparation:

[0039] Accurately weigh 2g of the mixed sample into a 150mL flat-bottomed flask, add 20mL of distilled water, 30mL of absolute ethanol, 1g of ascorbic acid, 1g of BHT, 20mL of 50% KOH aqueous solution, heat in a water bath at 80°C for 30min, cool to room temperature after completion, and use 50% Dilute ethanol water solution to 100mL, take 2mL through a 0.45μm filter membrane, and analyze on the machine.

[0040] 2. Set the instrument parameters:

[0041] The operating conditions of the online solid phase extraction part are:

[0042] (1) The vitamin D enrichment and purification column adopts PLRP-S column, the specification is 4.6×12.5mm, and the pore size is 15-20um;

[0043] (2) The mobile phase is 40% ethanol aqueous solution, and the running time is 22min; the mobile phase flow rate: 0-4min is 1mL / min; 4-15min is 0.2mL / min; 15-22min is 1mL / min, and the injection volume i...

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Abstract

The invention relates to a method for measuring vitamin D in milk powder. The method comprises following steps: 1), sample treatment: a milk powder sample is mixed uniformly, a proper quantity of a mixture is weighed, water, anhydrous ethanol, ascorbic acid, BHT and a KOH aqueous solution are added, heating and saponification are performed, and a solution obtained after saponification is cooled tobe in constant volume; 2), whether the solution obtained in the step 1) contains the vitamin D is measured by an HPLC (high performance liquid chromatograph) provided with a double-valve and three-pump system, and when two-dimensional liquid chromatography displays that the sample is suspected of containing vitamin D, the retention time of the sample is compared with that of a vitamin D standardsubstance so as to determine whether the sample contains the vitamin D; 3), if the sample contains the vitamin D, the content of the vitamin D in the sample is calculated according to a formula. Compared with the prior art, the method has the advantages that operating steps are simplified, the pretreatment time is greatly shortened, manpower and cost are saved, automatic online purification detection is realized, sensitivity is improved, and accuracy and reproducibility of a test result are guaranteed.

Description

[technical field] [0001] The invention belongs to the technical field of analytical chemistry, in particular to a method for determining vitamin D in milk powder. [Background technique] [0002] Vitamin D is an essential nutrient for the human body, and in its natural state, sunlight exposure or plant foods are important sources of vitamin D. Vitamin D is a steroid derivative. The most important members of its family are vitamins D2 and D3, which can regulate the metabolism of calcium and phosphorus in the human body and promote bone health. For example, the lack of vitamin D in the human body can lead to rickets in children, softening of bones and other diseases, while excessive content can cause calcium increase, and long-term use can easily cause symptoms such as firmness. Due to the limitation of lifestyle, the vitamin D synthesized by the body itself is difficult to meet the body's needs, so it must be supplemented through the food chain. Vitamin D is often used to fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/74G01N30/88G01N30/60
CPCG01N30/02G01N30/06G01N30/60G01N30/74G01N30/88G01N2030/027G01N2030/062G01N2030/884
Inventor 王卫孔祥芳陈建彪关玲玲
Owner SGS STANDARD TECHNICAL SERVICES SHANGHAI CO LTD
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