Method for inducing rapid callusing of phoebe zhennan

A fast and genus-sustaining technology, applied in the field of plant tissue culture in garden plant biotechnology, can solve the problems affecting the development and utilization of genus genus, low survival rate and small reproduction coefficient, and achieve high morphogenesis ability and induced differentiation ability, Accelerate the breeding of fine breeds, the effect of bright leaves

Active Publication Date: 2018-10-16
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current propagation methods of Zhennan are mainly sowing and cutting, but the reproduction coefficient is small, the survival rate is not high, and the growth is slow, which is far from meeting the market demand, which seriously affects the development and utilization of Zhennan.

Method used

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  • Method for inducing rapid callusing of phoebe zhennan
  • Method for inducing rapid callusing of phoebe zhennan
  • Method for inducing rapid callusing of phoebe zhennan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1), collection and cleaning of explants

[0034] The mother tree collected for explants is a one-year-old Zhennan seedling introduced from the Sichuan Academy of Forestry Sciences and planted in the Nongcui Garden of Anhui Agricultural University in March 2017. The new shoots germinated from the roots were selected, and then the branches were selected for growth. Robust, bright leaf color without spots, and young leaves free from diseases and insect pests are used as explants. Spray a little pure water + NAA0.1mg / L + grape seed extract proanthocyanidin 0.05g / L water-retaining agent on the surface of the young leaves for 5 minutes. Pick young leaves and bring them back to the laboratory to clean the explants. First, weigh 0.25g of washing powder and washing liquid and dissolve them in 250mL of water to obtain a cleaning solution. Then soak the explants in the cleaning solution for 10 minutes. Then carefully clean the surface of the explants, gently touch and wash the fr...

Embodiment 2

[0042] (1), collection and cleaning of explants

[0043] The mother tree collected for explants is the one-year-old Zhennan seedlings introduced from the Sichuan Academy of Forestry Sciences and planted in the Nongcui Garden of Anhui Agricultural University in March 2017. The newly grown branches from the top tips were selected, and then selected from the branches. The young leaves that grow vigorously, have bright leaf color without spots, and are free from diseases and insect pests are used as explants. Spray a little pure water + NAA2.5mg / L + grape seed extract proanthocyanidin 0.1g / L water retaining agent on the surface of the young leaves for 7 minutes. , take it back to the laboratory for explant cleaning, first weigh 0.25g of washing powder and washing liquid and dissolve it in 250mL of water to obtain a cleaning solution, then soak the explant in the cleaning solution for 15min, and then carefully wash the explant On the surface of the implant, gently touch and wash th...

Embodiment 3

[0051] (1), collection and cleaning of explants

[0052] The mother tree collected for explants is the one-year-old Zhennan seedlings introduced from the Sichuan Academy of Forestry Sciences and planted in the Nongcui Garden of Anhui Agricultural University in March 2017. The newly grown branches from the top tips were selected, and then selected from the branches. The young leaves that grow vigorously, have bright leaf color without spots, and are free from diseases and insect pests are used as explants. Spray a little pure water + NAA5.0mg / L + grape seed extract proanthocyanidin 0.2g / L water-retaining agent on the surface of the young leaves for 10 minutes. , take it back to the laboratory for explant cleaning, first weigh 0.25g of washing powder and washing liquid and dissolve it in 250mL of water to obtain a cleaning solution, then soak the explant in the cleaning solution for 20min, and then carefully wash the explant On the surface of the implant, gently touch and wash t...

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Abstract

The invention relates to a method for inducing rapid callusing of phoebe zhennan. The method comprises the following steps: by taking tender phoebe zhennan leaves as explants, collecting the explants,cleaning, sterilizing, cutting, inoculating, and carrying out dark culture and light culture, thereby completing rapid induced callusing of the phoebe zhennan. The callusing induction rate is up to 98.1% at most, a remarkable greening degrees and a callusing growth amount are achieved, and bases and related technical support can be provided for future phoebe zhennan callus induction, tissue culture for nursery stock reproduction, stock breeding acceleration and market requirement meeting.

Description

technical field [0001] The invention relates to the field of plant tissue culture in garden plant biotechnology, in particular to a method for inducing rapid healing of zhennan. Background technique [0002] Zhennan, also known as Nanmu, Jinsinan or Xiaoyenan, is a slow-growing evergreen tree in the genus Phoebe of Lauraceae with a height of more than 40 meters. It grows slowly. The large trees of nearly a thousand years have no signs of aging. It is a national second-level key protection species. Phoebe zhennan bark is smooth, light gray-brown, because of its slow growth, the prismatic or cylindrical green branchlets are generally thinner, covered with gray-brown hairs, and the old branches have no hairs; the leaves are leathery, the young leaves are red, the old leaves are green, the apex is acuminate, The base is wedge-shaped, the upper surface of the leaf is bright and glabrous, the lower part is densely pubescent, the leaf veins are longer, the ventral midrib is sunken...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 刘桂华余云云许早时邓波赵惠岳祥华雷刚詹文勇周立琪
Owner ANHUI AGRICULTURAL UNIVERSITY
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