Method for rapidly simultaneously detecting eight sweeteners and urethane in baijiu by UPLC serially connecting QDa

Abstract

The invention discloses a method for rapidly simultaneously detecting eight sweeteners and urethane in baijiu by UPLC serially connecting QDa. The method comprises the steps of pre-treating a baijiu sample to be tested, separating by ultra-high performance liquid chromatography, and carrying out data acquisition and detection with dual channels of a QDa detector. The method is simple, rapid, accurate and reliable, and can be used for simultaneously detecting the content of 8 sweeteners (acesulfame, sodium saccharin, cyclamate, aspartame, neotame, rebaudioside A, stevioside, and sucralose) andurethane in baijiu.

Description

technical field [0001] The invention relates to a method for quickly detecting eight kinds of sweeteners and ethyl carbamate in liquor by using UPLC in series with QDa, and belongs to the technical field of wine analysis. Background technique [0002] Baijiu, as a traditional Chinese beverage, has a history of thousands of years and has been widely inherited. During the fermentation process of liquor, a kind of ethyl carbamate will be produced, but once the ethyl carbamate produced in this environment is directly absorbed and drunk by the human body, it will seriously cause cancer in the body. The mellow and sweet taste of liquor is deeply loved by consumers. However, many wine companies add sweeteners to improve the taste for their own interests, and some companies use wine flavors and fragrances containing sweeteners due to lax purchase of raw materials, resulting in sweetness in liquor products. Dosage exceeded. Frequent reports of this phenomenon in the media have cau...

AI Technical Summary

Problems solved by technology

Studies have shown that the intake of high doses of sweeteners can cause damage to the human liver and nervous system, and even cause cancer. Therefore, the analysis and detection of sweeteners and ethyl carbamate is not only related to the quality of products, but also to consumers. health and safety are inseparable

[0003] At present, the general detection method of ethyl carbamate is the combination of chromatography and different detectors or the combination of chromatography and mass spectrometry. Among them, the sample pretreatment before detection includes liquid-liquid extraction, solid phase extraction and other forms. The detection of sweeteners is mainly Concentrate on gas chromatography, liquid chromatography and liquid chromatography tandem mass spectrometry, but both the detection of urethane and the detection of sweeteners require the use of multiple methods to complete the detection of these 9 substances, and Sometimes it cannot be detected accurately, and there are false positive judgments, which cause difficulties for relevant inspection departments

In addition, the limit values ​​of sweeteners and ethyl carbamate in wine are low. Using the original high performance liquid chromatography and gas chromatography methods cannot meet the low limit test requirements for exports.

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