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Method for utilizing ultra-effective bonded phase chromatography to serially connect QDa while quickly detecting seven biogenic amines in white spirit

A technology of ultra-efficient confluence and biogenic amines

Active Publication Date: 2016-08-17
ANHUI GUJING DISTILLERY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the colorimetric method can only be semi-quantitative, the liquid chromatography-derivatization method is unstable, the operation is time-consuming and troublesome, and the derivatization reagent benzoyl chloride is toxic, which is not conducive to human health
It has been reported in the literature that the tandem quadrupole method is used to test biogenic amines, but due to the small molecular weight of biogenic amines, there are no fragments with high abundance, and the fragments are too small, and the detection results are easily interfered
[0004] In addition, the limit value of biogenic amines in wine is low, and the original high-performance liquid phase derivatization method cannot meet the low limit test requirements for export

Method used

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  • Method for utilizing ultra-effective bonded phase chromatography to serially connect QDa while quickly detecting seven biogenic amines in white spirit
  • Method for utilizing ultra-effective bonded phase chromatography to serially connect QDa while quickly detecting seven biogenic amines in white spirit
  • Method for utilizing ultra-effective bonded phase chromatography to serially connect QDa while quickly detecting seven biogenic amines in white spirit

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Embodiment Construction

[0050] Below in conjunction with example the present invention is described further.

[0051] The present embodiment measures 7 kinds of biogenic amines in a liquor according to the following steps:

[0052] The instruments used are: ultra-high performance convergence chromatograph (equipped with QDa detector); BEH C 18 Composite chromatographic column (3.0×100mm, 1.7μm); Vortex instrument; Pipette gun.

[0053] The specific steps are:

[0054] (1) Pretreatment of the liquor sample to be tested:

[0055] Accurately measure 1.0 mL of the liquor sample to be tested in a 10 mL plastic centrifuge tube, add 9 mL of acetonitrile solution with a volume concentration of 80%, vortex for 5 min, centrifuge at 1000 rpm for 10 min, filter with a 0.22 μm filter membrane needle filter, and obtain the Sample to be tested; sample to be injected;

[0056] (2) Condition setting

[0057] The conditions for setting the ultra-high performance convergence chromatograph are:

[0058] The chromato...

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Abstract

The invention discloses a method for utilizing an ultra-effective bonded phase chromatography to serially connect QDa while quickly detecting seven biogenic amines in white spirit and belongs to the technical field of detection. The method is characterized by the following steps: after extracting a to-be-detected wine sample with acetonitrile, taking BEH C18 bonded phase chromatographic column as a separating column and carbon dioxide (A)+ isopropanol (B) as flowing phase for performing gradient eluting, and adopting a QDa mass spectrometry detector for detecting after separating the sample through the ultra-effective bonded phase chromatography (UPC2). The method provided by the invention is simple, quick, accurate, reliable and applicable to the simultaneous detection for the contents of seven biogenic amines (histamine, putrescine, cadaverine, phenylethylamine, spermidine, tyramine and octopamine) in white spirit.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for simultaneously rapidly detecting seven biogenic amines in liquor by using ultra-high-efficiency convergence chromatography connected in series with a QDa detector, and belongs to the technical field of wine analysis. Background technique [0002] Biogenic amines are biologically active substances, mainly including β-phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermidine, etc., which exist in a variety of foods, especially fermented foods (such as cheese, wine, etc.) , beer, rice wine, fermented sausage, condiments), aquatic products and meat products, etc. Biogenic amines, especially histamine, are inherently toxic, so they are a major indicator to measure the freshness of meat and aquatic products and the hygiene conditions in the wine production process. [0003] The current GB standard is detected by colorimetric method or liquid ...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88G01N33/14
CPCG01N30/06G01N30/88G01N33/146G01N2030/027G01N2030/065G01N2030/8809
Inventor 王银辉刘国英汤有宏沈小梅马雷李安军
Owner ANHUI GUJING DISTILLERY
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