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A method for simultaneous and rapid detection of six sugars in health wine by using ultra-high performance liquid chromatography in series with QDA

An ultra-high performance liquid phase and sugar technology, applied in the field of detection and analysis, can solve problems such as taking hours to reach equilibrium, unable to achieve sensitivity and selectivity, changing mobile phase components, etc., to achieve excellent separation effect and reduce detection. Good effect of cost and standard recovery

Active Publication Date: 2020-05-22
ANHUI GUJING DISTILLERY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With RI detection, it is difficult to change the mobile phase composition from one analysis to the next because the RI detector can take hours to equilibrate when using different mobile phase compositions, even when using a batch of RI can still detect small changes that lead to baseline changes when using the same new mobile phase
In the case of changes in mobile phase components, ELS detection is relatively more reliable, but ELS often cannot achieve the sensitivity and selectivity required for the detection of sugars in complex food matrices. Substance-like approach looms

Method used

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  • A method for simultaneous and rapid detection of six sugars in health wine by using ultra-high performance liquid chromatography in series with QDA
  • A method for simultaneous and rapid detection of six sugars in health wine by using ultra-high performance liquid chromatography in series with QDA
  • A method for simultaneous and rapid detection of six sugars in health wine by using ultra-high performance liquid chromatography in series with QDA

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Embodiment Construction

[0052] The technical solutions of the present invention will be further elaborated below in conjunction with specific embodiments.

[0053] 1. Pretreatment

[0054]Measure 5.0 mL of the health wine sample to be tested into a 10 mL plastic centrifuge tube, add 10 mL of acetonitrile solution with a volume concentration of 75%, vortex for 2 min, centrifuge at 1000 rpm for 5-10 min, and filter with a 0.22 μm membrane syringe filter. Obtain the sample to be tested;

[0055] 2. Detection condition setting

[0056] The detection conditions of the ultra-high performance liquid chromatography are set as follows:

[0057] Chromatographic column: 2.1×150mm, 1.7μm BEH Amide carbohydrate analysis column; vortex instrument and pipette gun;

[0058] The column temperature is 40°C;

[0059] The temperature of the sample chamber is 15°C;

[0060] Mobile phase: A phase is 75% acetonitrile aqueous solution (A), B phase is 10mmolNH 4 HCO 3 solution (containing 0.1% NH 4 OH solution);

[...

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Abstract

The invention discloses a method for quickly detecting six carbohydrate substances at the same time by utilizing an ultra-high performance liquid chromatography cascading QDa. The method comprises thesteps: after preprocessing a wine sample to be detected, utilizing the ultra-high performance liquid chromatography provided with a QDa detector to detect the wine sample to obtain a chromatogram ofthe sample to be detected; performing qualitative analysis according to chosen ions; then performing qualitative analysis on a health-care wine sample to be detected according to standard curves of the six carbohydrates. The method disclosed by the invention has the advantages of simpleness, quickness, convenience, accuracy and reliability, application to detecting contents of the six carbohydratesubstances (glucose, fructose, saccharose, lactose, maltose and inositol) in the health-care wine at the same time and ability in providing scientific basis for accurately judging and quickly detecting carbohydrates in the health-care wine.

Description

technical field [0001] The invention relates to a method for quickly detecting six carbohydrates in health wine by using ultra-high performance liquid chromatography connected in series with QDa, and belongs to the technical field of detection and analysis. Background technique [0002] Both sugars and sugar alcohols are carbohydrates, which are natural components in food and health wine, and have important nutritional value. Some sugars are added to processed foods to add flavor or to mimic fresh foods. In recent years, with the increasing prevalence of obesity and diabetes in developed countries, the need for further monitoring of sugar intake has increased. Therefore, in order to meet increasingly stringent regulatory requirements, accurate sugar content information needs to be provided on food labels. As reported in the literature, the analysis of sugars and sugar alcohols is extremely challenging due to the lack of chromophores in their compound structures and the clo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 王银辉高家坤崔玉军刘国英
Owner ANHUI GUJING DISTILLERY
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