Silica nanowire array chip for enrichment and detection of circulating tumor cells in whole blood, and preparation method of silica nanowire array chip

A nanowire array and silicon dioxide technology, which is applied in the field of biological materials and clinical detection, can solve the problems of little research progress, long capture time, and low efficiency, and achieve the effects of improving capture efficiency, simple operation, and high sensitivity

Inactive Publication Date: 2018-10-09
BEIJING SCITECH NANOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods still have disadvantages such as low efficiency, low purity and long capture time.
Although the combination of two or three methods may become a more ideal method, there is little research progress in the past ten years

Method used

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  • Silica nanowire array chip for enrichment and detection of circulating tumor cells in whole blood, and preparation method of silica nanowire array chip
  • Silica nanowire array chip for enrichment and detection of circulating tumor cells in whole blood, and preparation method of silica nanowire array chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The nanowire length of the silicon dioxide nanowire array chip prepared in this example is 0.3 μm; taking circulating tumor cells EpCAM specific cells and EpCAM non-specific cells as circulating tumor cells to be captured as examples, the capture system of the present invention was used as an example. Further elaboration and verification. The method for specifically capturing circulating tumor cells by using the micro-nano structure on the surface of the silica nanowire array and the specific recognition molecule to cooperate includes the following steps:

[0035] (1) In the n-pentanol system, blend and stir polyvinylpyrrolidone (PVP, 20g / L), water and ethanol to form a water-in-oil microemulsion, wherein the ratio of n-pentanol, water and ethanol is 1:0.01 :0.01;

[0036] (2) The PDMS substrate is subjected to hydrophilic treatment;

[0037] (3) The microemulsion prepared by step (1) is uniformly adsorbed on the substrate of step (2), and at room temperature, it is p...

Embodiment 2

[0049] The nanowire length of the silicon dioxide nanowire array chip prepared in this example is 1.5 μm; taking circulating tumor cells EpCAM specific cells and EpCAM non-specific cells as circulating tumor cells to be captured as examples, the capture system of the present invention was used as an example. Further elaboration and validation. The method for specifically capturing circulating tumor cells by using the micro-nano structure on the surface of the silica nanowire array and the specific recognition molecule to cooperate includes the following steps:

[0050] (1) In the n-pentanol system, blend and stir polyvinylpyrrolidone (PVP, 300g / L), water and ethanol to form a water-in-oil microemulsion, wherein the ratio of n-pentanol, water and ethanol is 1:0.01 :0.01;

[0051] (2) The PDMS substrate is subjected to hydrophilic treatment;

[0052] (3) The microemulsion prepared by step (1) is uniformly adsorbed on the substrate of step (2), and at room temperature, it is pl...

Embodiment 3

[0064] The nanowire length of the silicon dioxide nanowire array chip prepared in this embodiment is 10 μm; taking circulating tumor cells EpCAM specific cells and EpCAM non-specific cells as the circulating tumor cells to be captured as an example, the capture system of the present invention is further described Elaborate and verify. The method for specifically capturing circulating tumor cells by using the micro-nano structure on the surface of the silica nanowire array and the specific recognition molecule to cooperate includes the following steps:

[0065] (1) In the n-pentanol system, mix and stir polyvinylpyrrolidone (PVP, 20g / L), water and ethanol to form a water-in-oil microemulsion, wherein the ratio of n-pentanol, water and ethanol is 1:3 :3;

[0066] (2) The PDMS substrate is subjected to hydrophilic treatment;

[0067] (3) The microemulsion prepared by step (1) is uniformly adsorbed on the substrate of step (2), and at room temperature, it is placed under the atm...

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Abstract

The invention relates to a silica nanowire array chip which grows on a polydimethylsiloxane interface and realizes enrichment and detection of circulating tumor cells in whole blood. In an organic phase system, water-in-oil microemulsion is prepared by using the difference in solubility and is assembled on the polydimethylsiloxane interface, so that the in-situ growth of a silica nanowire is catalyzed and promoted, and a silica nanowire array chip is obtained; specific antibodies on the surfaces of the circulating tumor cells are then immobilized on the silica nanowire, so that a silica nanowire array chip having the specific antibodies immobilized on the surfaces of the circulating tumor cells is obtained; a whole blood sample, which is to be tested and contains the circulating tumor cells, is dropwise added onto the surface of the chip, and the specific antibodies on the surfaces of the circulating tumor cells have a synergistic effect with the nanowire structure, so that the circulating tumor cells in the whole blood to be tested can be specifically detected.

Description

technical field [0001] The invention relates to the field of biological materials and clinical detection, in particular to a silicon dioxide nanowire array chip for capturing cancer cells in whole blood and a preparation method thereof, in particular to a silicon dioxide nanowire array chip for enrichment and detection of circulating tumor cells in whole blood Line array chip and its preparation method. Background technique [0002] Tumor recurrence and metastasis is the main cause of death. Detection of circulating tumor cells (circulating tumor cells, CTCs) in peripheral blood has important application value for solving clinical problems such as recurrence and metastasis monitoring, tumor molecular characteristics and so on. Circulating tumor cells (CTCs) refer to tumor cells that enter the peripheral blood circulation from primary tumors or metastases due to spontaneous or therapeutic procedures. Most tumor cells that invade the circulatory system die within a short per...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N5/09C01B33/16B82Y40/00
CPCB82Y40/00C01B33/163C01P2004/16C12M45/00C12N5/0694C12N2509/00
Inventor 孟靖昕李冠男王树涛江雷
Owner BEIJING SCITECH NANOTECH CO LTD
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