T cell vaccine constructed by secretory component of gene engineering-based aAPC (artificial Antigen Presenting Cell) as well as preparation method and application thereof
A technology of genetic engineering and cell secretion, which is applied in the field of preparation of T cell vaccines and can solve the problems of lack of endogenous expression
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[0039] The present invention shows that activated non-specific T cells can acquire vesicles derived from genetically engineered artificial antigen-presenting cells, in particular, these cells can acquire antigen-specific histocompatibility complexes and co-stimulatory molecules from the vesicles. The present invention shows that these vesicle-derived molecules are functional. In this way, non-specific T cells targeted by vesicles can directly stimulate antigen-specific immune responses.
[0040] The present invention shows that the genetically engineered artificial antigen-presenting cells are derived from K562 cells that lack endogenously expressed HLA-A, B and DR histocompatibility conformers. K562 cells were first expressed by two eukaryotic expression plasmid vectors pcDNA Hygro / HLA-A2 and
[0041] pcDNA Neo / CD80 transfection. AdV Gag and AdV 41BBL Transfection to form genetically engineered antigen-presenting cells K562 expressing transgenic HLA-A2, CD80, Gag and 41...
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