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Molecular marker closely linked with wheat stem height and spike length character major QTL and application thereof

A technology of wheat and ear length, applied in the field of genetic breeding at the molecular biology level, to achieve the effect of speeding up the breeding process, clear location, and large field error in identification

Active Publication Date: 2018-09-28
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on map-based cloning of wheat plant height and panicle length QTL

Method used

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  • Molecular marker closely linked with wheat stem height and spike length character major QTL and application thereof
  • Molecular marker closely linked with wheat stem height and spike length character major QTL and application thereof
  • Molecular marker closely linked with wheat stem height and spike length character major QTL and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1, construction of wheat plant height positioning population and segregation population and character determination

[0055] The population used in this example is the RIL population derived from Yumai 8679 and Jing 411 and the F 2 population (F derived from a remaining heterozygous line yj-171 in the RIL population 2 group.

[0056] Both the RIL population and the heterozygous line yj-171 are described in the following literature: Zhai HJ, Feng ZY, Li J, Liu XY, Xiao SH, Ni ZF, Sun QX (2016) QTL Analysis of Spike Morphological Traits and Plant Height in Winter Wheat (TriticμM aestivμM L.) Using a High-Density SNP and SSR-Based Linkage Map. Frontiers in Plant Science 7. doi:10.3389 / fpls.2016.01617. Plant height phenotype data for RIL populations can also be obtained from this literature, F 2 Population phenotype data are measured.

[0057] yj-171 derived F 2 F 2 group.

[0058] 1. Extraction of total DNA from leaves at seedling stage

[0059] (1) Take...

Embodiment 2

[0080] Example 2, Application of Molecular Marker STARP-2001 in Dwarf Breeding

[0081] 1. Planting F of Yumai 8679 and Jing 411 in the field 2 group.

[0082] 2. List samples at the seedling stage, extract the total DNA of the leaves, and perform PCR amplification on it using the molecular marker STARP-2001. The PCR amplification system is as shown in Table 1, and the PCR amplification reaction is as shown in Table 2.

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Abstract

The invention discloses a molecular marker closely linked with wheat stem height and spike length character major QTL and application thereof. A primer pair used for detecting stem height or spike length of to-be-detected wheat is provided and is used for amplifying target segments; the target segments contain segments of obtained segments generated by amplification of a primer1, primer 2 and primer 3; the primer 1 is a single chain DNA molecule shown in sequence 1, the primer 2 is a single chain DNA molecule shown in sequence 2; the primer 3 is a single chain DNA molecule shown in sequence 3;the experiment proves that the primer pair STARP-2001 is utilized to carry out assistant selection on wheat segregation groups. The result shows that a single plant with genotype being consistent with high stem parent Jing 411 is significantly higher than a single plant with the genotype being consistent with short stem parent, and the primer pair STARP-2001 used for molecular marker assistant selection of wheat short stem breeding is practical and effective.

Description

technical field [0001] The invention belongs to the technical field of molecular biology-level genetic breeding, and in particular relates to a molecular marker closely linked with the main effect QTL of wheat plant height and ear length traits and an application thereof. Background technique [0002] Wheat is the second largest ration crop in my country. According to China Grain and Oils Information Network, the national wheat supply in 2013 / 14 was about 220.8 billion catties, the demand was about 232.8 billion catties, and the annual gap was about 12 billion catties. The domestic wheat supply and demand are still in a tight balance with a slight gap. my country's new strategic goal of food security is to ensure that the self-sufficiency rate of the three major staple foods of rice, wheat, and corn remains above 95%, and the two major rations of rice and wheat maintain 100% self-sufficiency. As the population continues to increase and the area of ​​cultivated land decrease...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 倪中福柴岭岭孙其信翟会杰陈朝燕彭惠茹肖世和
Owner CHINA AGRI UNIV
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