Rare earth nucleoside fluorescent hydrogel, preparation method and application in fluorescent coding

Abstract

The invention discloses rare-earth nucleoside fluorescent hydrogel as well as a preparation method and application thereof in fluorescent encoding. The preparation method of the rare-earth nucleosidefluorescent hydrogel comprises the following steps: (1) preparing a soluble rare-earth nitrate water solution and a nucleoside water solution; (2) uniformly mixing the soluble rare-earth nitrate watersolution and the nucleoside water solution and regulating the pH (Potential of Hydrogen) value to 7 to 9; raising the temperature to 55 to 95 DEG C and standing and reacting for 10 to 20min; coolingto room temperature and standing for 1 to 4h to obtain the rare-earth nucleoside fluorescent hydrogel with different fluorescent colors. The rare-earth nucleoside fluorescent hydrogel disclosed by theinvention has the advantages of environment-friendly and safe raw materials, simplicity in operation, low cost and good repeatability; the rare-earth nucleoside fluorescent hydrogel disclosed by theinvention keeps characteristic of emission fluorescent light of rare-earth ions and also has characteristics of good biocompatibility of biological small molecular nucleoside, biodegradability, no toxicity and the like. The obtained fluorescent hydrogel is regulated and controlled to obtain various fluorescent colors and can be used as a fluorescent encoding material; the fluorescent hydrogel canbe used for fluorescent biological detection, cell culture and tissue engineering.

Description

technical field [0001] The invention belongs to the field of fluorescent hydrogel, and specifically relates to a fluorescent hydrogel, a preparation method and an application in fluorescent coding. Background technique [0002] Fluorescent hydrogels are widely used in various fields such as fluorescent biodetection, cell culture, and tissue engineering. Rare earth fluorescent hydrogels not only have the excellent photophysical properties of rare earths, such as: high quantum yield, long fluorescence lifetime, narrow emission band, large Stokes shift, etc.; they also have unparalleled biological application characteristics of hydrogel materials. , has been widely concerned by researchers. However, during the assembly process of rare earth fluorescent hydrogels, due to the competitive coordination between water molecules and ligand molecules in the aqueous solution, the fluorescence of rare earth ions will be quenched, which greatly affects the rare earth fluorescent water. ...

AI Technical Summary

Problems solved by technology

[0004] At present, most rare earth-based fluorescent encoding materials are concentrated in rare earth upconversion materials, and it is still a challenge to introduce nucleoside rare earth complexes into fluorescent hydrogels with fluorescent encoding functions through regulation.

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