Method for preparing diglyceride through enzyme catalysis of ester exchange of monoglyceride and medium chain triglyceride

Abstract

The invention belongs to the technical field of grease deep processing, and particularly relates to a method for preparing diglyceride through enzyme catalysis of ester exchange of monoglyceride and medium chain triglyceride. The method comprises the following steps that long chain fatty acid monoglyceride is heated and melted; then, a certain quantity of medium chain fatty acid triglyceride is added; uniform mixing is performed; the temperature is regulated to 60 to 80 DEG C; lipase is added, wherein the adding quantity of the lipase is 3 to 9 percent of the total mass of a substrate; the ester exchange reaction is performed under the vacuum and stirring conditions; the ester exchange reaction time is 15 to 120min; a crude diglyceride product is obtained. The lipase is used for catalyzinglong chain fatty acid monoglyceride and performing ester exchange with medium chain fatty acid triglyceride to prepare a diglyceride containing grease base material under the rotary water bath condition. The method has the advantages that the process is simple; the cost is low; the raw materials can be easily obtained; good prospects are realized in the aspect of realizing the industrial production of diglyceride.

Description

technical field [0001] The invention belongs to the technical field of oil deep processing, and in particular relates to a method for preparing diglyceride by enzyme-catalyzed transesterification of monoglyceride and medium-chain triglyceride. Background technique [0002] Diacylglycerol (DAG) is a generally recognized as safe (GRAS) food ingredient, and its natural content in vegetable oil is generally between 1-6%, and it can reach 10% in cottonseed oil. They exist as two isomers, 1,3-DAG and 1,2-DAG, in a ratio of approximately 1:2 in thermal equilibrium. DAG is mainly used in the production of emulsifiers, stabilizers and as carriers in the fields of food, cosmetics and pharmaceuticals. DAG has similar digestibility and energy value to triglycerides (TAG). DAG-enriched oils have been extensively studied as an alternative to TAG-based oils due to their beneficial effects on lipid metabolism. Due to the structural difference between DAG and TAG, DAG has the function of ...

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the general preparation of diglycerides is mostly obtained by triglycerides through glycerololysis or hydrolysis; while fatty acids and glycerol are esterified to prepare diglycerides. If chemical methods are used, very high reaction temperatures are required. Energy saving and environmental protection. If the enzymatic method is also used, more expensive enzymes are needed to catalyze the esterification, which is poor in practicability, and there will be a large amount of unreacted free fatty acids in the product

However, there are few reports on the preparation of DAG by enzyme-catalyzed transesterification of MAG and TAG.

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