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Preparation method of Se-enriched lactobacillus

A lactic acid bacteria and selenium-enriched technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problem that the conversion rate of inorganic selenium and intracellular organic selenium content are not clearly given, and the lack of selenium-enriched lactic acid bacteria cultivation methods, etc. problems, to achieve the effect of easy cultivation scale, low cost and high yield

Pending Publication Date: 2018-09-07
SHAANXI UNIV OF SCI & TECH
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Problems solved by technology

The preparation of selenium-enriched lactic acid bacteria still has the following deficiencies: 1) The current research focuses on the fermentation of dairy products, and the conversion rate of inorganic selenium, the content of organic selenium in cells and the technological measures to improve the conversion rate of inorganic selenium are not clearly given, which is not enough for enriched lactic acid bacteria. The industrialized production of selenium inoculum provides practical basis; 2) also lacks the cultivation method of selenium-enriched lactic acid bacteria that meets industrialized production requirements at present

Method used

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Embodiment Construction

[0021] The present invention will be described in further detail below in conjunction with the examples.

[0022] In the present invention, the low-temperature-preserved Lactobacillus plantarum is inserted into the MRS broth medium for activation, and the optimal method for producing selenium-enriched Lactobacillus plantarum is determined by adjusting the pH value and the concentration of sodium selenite under a certain inoculation amount and culture temperature. fermentation conditions. The content of organic selenium in cells was determined by catalytic absorptiometry.

[0023] 1. Strains

[0024] Lactobacillus plantarum (Lactobacillus plantarum) comes from CICC, strain number 23941, and is preserved in the Natural Product Microbial Manufacturing Laboratory of Shaanxi University of Science and Technology.

[0025] 2. Medium

[0026] Composition of MRS broth liquid medium: peptone 10g / L, beef extract powder 5g / L, yeast extract powder 4g / L, glucose 20g / L, dipotassium hydrog...

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Abstract

The invention provides a preparation method of Se-enriched lactobacillus. The Se-enrichment degree of lactobacillus is increased by adopting fermentation and regulating conditions such as inorganic Seconcentration, pH value and the like in the fermentation process, Se content of cells of obtained lactobacillus plantarum is as high as 847.52 mu g / g, and the transformation rate of inorganic Se reaches 98.3%. The preparation method has the advantages of being low in cost, high in yield and short in fermentation cycle and facilitating scale-up culture.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for enriching selenium by Lactobacillus plantarum. Background technique [0002] Selenium has been jointly recognized by the United Nations Food and Agriculture Organization (Food and Agriculture Organization, FAO) / International Atomic Energy Agency (International Atomic Energy Agency, IAEA) / World Health Organization (World Health Organization, WHO) as an essential trace element for the human body, and it is essential for maintaining the body It is related to normal physiological function and prevention of various diseases, and is an element in the active center of various enzymes in mammals. [0003] Studies have shown that the selenium content in grains, vegetables, and fruits is low, which cannot meet the human body's demand for selenium (recommended dietary intake: 55 μg / d). Daily supplementation of selenium is mainly through inorganic selenium and o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/25
CPCC12N1/20
Inventor 钱卫东卢家兴王婷段冉冉沈兰芳刘婵婵刘淼
Owner SHAANXI UNIV OF SCI & TECH
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