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A strain of Pediococcus lactis hew-ap27 and its application

A technology of HEW-AP27 and Pediococcus lactis, applied in the field of probiotics, can solve problems such as susceptibility to infection, enhanced drug resistance of strains, and impact on animal immunity

Active Publication Date: 2021-04-20
BEIJING HESWOF BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the extensive use of antibiotics and synthetic antibacterial drugs will increase the amount of drug residues, increase the resistance of bacterial strains, affect the immunity of animals, and make them susceptible to infection

Method used

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  • A strain of Pediococcus lactis hew-ap27 and its application
  • A strain of Pediococcus lactis hew-ap27 and its application
  • A strain of Pediococcus lactis hew-ap27 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Isolation, screening, identification and preservation of Pediococcus lactis HEW-AP27

[0049] 1. Primary screening of strains

[0050] Take 1 g of the intestinal contents of healthy piglets and enrich them in 100 mL of MRS liquid medium for 8 hours, then treat the enriched culture solution in a water bath at 85°C for 30 minutes, and place the treated enriched culture solution on the MRS plate Carry out zoning and marking, incubate upside down in a 37°C incubator for 24-48 hours, observe the colony shape, pick colonies that grow well and have the typical colony shape of lactic acid bacteria for pure culture, and store them for future use.

[0051] 2. Re-screening of strains

[0052] According to the Gram-positive, catalase-negative and non-spore-producing strains can be initially identified as lactic acid bacteria, a total of 58 strains, respectively numbered as HEW-AP1, HEW-AP2, HEW-AP3, ... HEW-AP58, Filter as follows.

[0053] Only the performance of the ...

Embodiment 2

[0101] The preparation of embodiment 2 Pediococcus lactis preparation

[0102] Mix the Pediococcus lactis HEW-AP27 sludge obtained in Example 1 and the stabilizing and protecting agent in a mixing tank at a mass ratio of 0.8:1.2 for 20 minutes, mix well, and then adjust the humidity to 36% by adding water to obtain the bacteria wet powder Put the wet bacteria powder into the granulator for granulation, so that the particle size is 25 mesh, and then dry it through the swirling fluidized bed granulation coating machine, control the inlet air temperature at 60±2°C, and control the outlet air temperature at 32±2°C, dry for 1 hour; start the side spray coating device, and spray the coating liquid to the material in the swirl state at a speed of 20mL / min to form a coating film layer, control the inlet air temperature to 50±2°C, the mist Pressure is 0.15MPa, obtains 25 purpose Pediococcus lactis live bacteria preparations.

[0103] The stabilizing and protecting agent is composed of...

Embodiment 3

[0108] Application of Example 3 Pediococcus lactis preparation in the production of weaned piglets

[0109] In the experiment, 200 weaned piglets with similar body weight, gender (8.8±0.36) and the same age were selected and randomly divided into two groups (control group and test group), with 5 repetitions in each group and 20 piglets in each repetition. Feed the basal ration (formula as shown in Table 9); another group is the test group, fed the basal ration+Pediococcus lactis preparation (addition amount 10 6 CFU / kg piglet feed). The test period was 1 month. During the test period, all piglets had free access to food, free drinking water and routine immunization.

[0110] The fasting body weight of the piglets was weighed at the beginning and end of the experiment, the health, diarrhea and death of the piglets were observed every day, the feed consumption, diarrhea and death of the piglets were recorded, and the diarrhea rate, mortality rate, average daily feed intake, ave...

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Abstract

The present invention discloses a newly isolated strain of Pediococcus acidilactici (Pediococcus acidilactici) HEW‑AP27, which is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee (CGMCC for short, address: Yard 3, No. 1, Beichen West Road, Chaoyang District, Beijing. No. Institute of Microbiology, Chinese Academy of Sciences, postal code: 100101), the preservation number is CGMCC NO.15419, and the preservation date is March 7, 2018. The present invention also provides a preparation method of live Pediococcus lactis preparation, specifically comprising: using Pediococcus lactis as a fermentation strain, adopting an optimized fermentation process and a fermentation medium, and putting it into a tank after primary, secondary, and tertiary cultivation , centrifuge to obtain active bacteria slime, add stabilizer and protective agent, then granulate through a granulator, and then add a coating solution in a coating machine for coating, and finally obtain a viable Pediococcus lactis preparation. The Pediococcus lactis HEW‑AP27 of the present invention is acid-resistant, bile-salt-resistant, high-temperature-resistant, has strong acid-producing performance, and can also inhibit the growth and reproduction of pathogenic bacteria.

Description

technical field [0001] The invention relates to probiotics, in particular to a strain of Pediococcus acidilactici (Pediococcus acidilactici) HEW-AP27 and its application. Background technique [0002] Pediococcus acidilactici is one of the important members of probiotics, and most of them are one of the probiotics on the body surface and internal mucosal surface of animals. In addition, Pediococcus lactis also produces acid, which can regulate the gastrointestinal flora and maintain the intestinal microecological balance. It can antagonize pathogenic microorganisms in animals, competitively inhibit pathogenic microorganisms, enhance the immune function of animals, produce beneficial metabolites, activate the activity of acid protease, participate in the metabolism of the body, and prevent the production of harmful substances. [0003] At present, the abuse of antibiotics, chemically synthesized drugs, and hormones in feed and animal husbandry has caused very serious consequ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K50/30A23K50/60A23K50/75A23K10/18A23K40/30A23K40/10C12R1/01
CPCA23K10/18A23K40/10A23K40/30A23K50/30A23K50/60A23K50/75C12N1/20C12N1/205C12R2001/01
Inventor 李雪平杜志琳尹望刘建芳袁武易忠
Owner BEIJING HESWOF BIOTECH CO LTD
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