Method for detecting content of various kinds of ingredients in herba gerberae piloselloidis medicinal material

Abstract

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for detecting the content of various kinds of ingredients in herba gerberae piloselloidis medicinal material. The method adopts a UPLC-PDA method, takes Waters BET C18 (2.1*150mm, 1.7microns) as a chromatographic column, acetonitrile-0.1% formic acid water solution is subjected to gradient elution, the detection wavelength is 280nm and 328nm, the flowing velocity is 0.28L / min, and the column temperature is 40 DEG C. According to the method, chlorogenic acid, isochlorogenic acid A,isochlorogenic acid B, isochlorogenic acid C, galuteolin and arbutin in herba gerberae piloselloidis medicinal material can be well separated, the average recovery of the method is 97.20%-103.57%, andRSD (relative standard deviation) is less than or equal to 3.0%. The method is simple and convenient and precise, the repeatability is good, the method can be used for detecting the content of chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, galuteolin and arbutin in herba gerberae piloselloidis medicinal material, and reference foundations are provided for quality control of herba gerberae piloselloidis.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicines, in particular to a method for measuring the contents of various components in the herba basilica medicinal material. Background technique [0002] Gerbera japonicus is the dry whole herb of Piloselloides hirsuta hirsuta (Forsk.) G. Jeffrey of Compositae, and it is also used by ethnic minorities in Guizhou Province. Rhododendron is mild in nature, bitter and pungent, and returns to the lung and stomach meridians. It has the effects of clearing the lung and relieving cough, producing sweat and diuresis, promoting qi and blood circulation, and is mainly used for cold cough, asthma, edema, fullness of urination, dyspepsia in children, amenorrhea, Injuries from falls, carbuncles, furuncles and other diseases. According to the "Guiyang Folk Medicinal Herbs", gerbera is mainly used to treat lung-related diseases such as wind cough, cough asthma and lung abscess. Mode...

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Problems solved by technology

Rhizoma Rhododendron was included in the 2003 edition of "Quality Standards for Traditional Chinese Medicinal Materials and Ethnic Medicinal Materials in Guizhou Province" and the 2005 edition of "Quality Standards for Decoction Pieces of Traditional Chinese Medicine in Yunnan Province", but the content of each beneficial component was not determined. The quality standards are not perfect, and the internal quality of Maodadingcao medicinal materials cannot be comprehensively controlled

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