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Extracting method and application of armadillidiam vulgare phaseolin polypeptide

A protein polypeptide and extraction method technology, applied in the field of protein polypeptide extraction, can solve the problem of high cost and achieve the effects of mild conditions, easy realization and simple extraction

Inactive Publication Date: 2018-09-04
FOSHAN HUIMU CHEM TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are few related reports on the extraction of murine protein polypeptides, but it can be seen from the only patent: Chinese patent CN201610920569.9 A kind of murine fibrinolytic active protein and its application did not undergo degreasing treatment, but adopted In the later stage, after multiple chromatography such as ion exchange and gel filtration, the active protein is obtained by ultrafiltration and concentration, and the cost is relatively high; the same patent also has a Chinese patent CN201510042710. is the traditional method, by using ammonium sulfate for protein precipitation followed by dialysis to remove ammonium sulfate

Method used

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  • Extracting method and application of armadillidiam vulgare phaseolin polypeptide
  • Extracting method and application of armadillidiam vulgare phaseolin polypeptide
  • Extracting method and application of armadillidiam vulgare phaseolin polypeptide

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for extracting protein polypeptides from muskwort, comprising the steps of:

[0026] (1) After starvation treatment for 7.5 hours, then wash, dry, and pulverize through a 70-mesh sieve to obtain murine powder;

[0027] (2) Take the molasses powder in step (1), add petroleum ether with a mass volume ratio of 2, ultrasonically extract for 25 minutes, filter, and dry in an oven at 40°C to obtain defatted molasses powder;

[0028] (3) Take the degreased muffin powder of step (2), add 10% hydrochloric acid solution with a mass volume ratio of pH=2, then add pepsin, stir and incubate at 36.5°C for 4h, adjust the pH value to 2 every 1h, filter, Collect the filtrate;

[0029] (4) Centrifuge the filtrate of step (3) at 3200 r / min for 18 min, take the supernatant, concentrate at low temperature in vacuum and freeze-dry in vacuum to obtain murine protein polypeptide.

Embodiment 2

[0031] A method for extracting protein polypeptides from muskwort, comprising the steps of:

[0032] (1) After starvation treatment for 10 hours, then wash, dry, and pulverize through an 80-mesh sieve to obtain a sieve powder;

[0033] (2) Take the molasses powder in step (1), add petroleum ether at a mass volume ratio of 3, extract it ultrasonically for 30 minutes, filter it, and dry it in an oven at 45°C to obtain degreased molasses powder;

[0034] (3) Take the degreased muffin powder of step (2), add 12% hydrochloric acid solution with a mass volume ratio of pH=2, then add pepsin, stir and incubate at 38°C for 5h, adjust the pH value to 2.2 every 1h, filter, Collect the filtrate;

[0035] (4) Centrifuge the filtrate of step (3) for 20 min at 3500 r / min, take the supernatant, concentrate at low temperature in vacuum and freeze-dry in vacuum to obtain murine protein polypeptide.

Embodiment 3

[0037] A method for extracting protein polypeptides from muskwort, comprising the steps of:

[0038] (1) After starvation treatment for 5 hours, then wash, dry, and pulverize through a 60-mesh sieve to obtain murine powder;

[0039] (2) Take the molasses powder in step (1), add petroleum ether with a mass volume ratio of 1, ultrasonically extract for 20 minutes, filter, and dry in an oven at 35°C to obtain degreased molasses powder;

[0040] (3) Take the degreased muffin powder of step (2), add 8% hydrochloric acid solution with a mass volume ratio of pH = 2, then add pepsin, stir and incubate at 35°C for 3h, adjust the pH value to 1.8 every 1h, filter, Collect the filtrate;

[0041] (4) Centrifuge the filtrate of step (3) for 15 min at 3000 r / min, take the supernatant, concentrate at low temperature in vacuum and freeze-dry in vacuum to obtain murine protein polypeptide.

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Abstract

The invention discloses an extracting method and application of armadillidiam vulgare phaseolin polypeptide, and further provides liquid feed containing the phaseolin polypeptide. The extracting process of the armadillidiam vulgare phaseolin polypeptide roughly comprises the steps of cleaning treatment, crushing degreasing, enzymolysis extraction and freeze drying. The armadillidiam vulgare phaseolin polypeptide is prepared through the extracting method, the process is simpler than that of other patents, and impurities are not introduced so that the high quality retentivity can be achieved.

Description

technical field [0001] The invention relates to a method for extracting protein polypeptides, in particular to a method for extracting protein polypeptides from ratwort. Background technique: [0002] It is the whole worm of the Isopoda Toribetidae animal torii or the Mosquito, also known as the squirrel, the squirrel, the wet worm, the ground chicken, the wet worm, etc. It is the only complete insect in the Crustacea. Animals adapted to life on land. [0003] The main function of edible protein is to supplement the protein obtained after hydrolysis in food and beverages, which can supplement the human body with easily absorbed compound protein and amino acids, and can effectively improve the nutritional structure of the human body, especially to make the nutritional structure of protein substances reach a balanced ratio. , so as to indirectly achieve the effect of enhancing physical fitness, promoting development and reducing disease. [0004] There are few related report...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14A23K20/147
CPCA23K20/147C07K1/145C12P21/06
Inventor 不公告发明人
Owner FOSHAN HUIMU CHEM TECH CO LTD
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