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Bioactive polysaccharose dressing for wound restoration as well as preparation method and application thereof

A bioactive polysaccharide and bioactive technology, which is applied in the field of bioactive polysaccharide dressings and its preparation, can solve the problems of PDGF-BB loss and poor dose controllability, and achieve the effect of convenient operation, convenient application, and promotion of fibroblast migration

Inactive Publication Date: 2018-08-14
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of the wound environment, externally applied PDGF-BB has a large degree of degradation and loss, and the dose controllability is poor, and there are still unknown biological risks in the large-scale application of exogenous PDGF-BB

Method used

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  • Bioactive polysaccharose dressing for wound restoration as well as preparation method and application thereof
  • Bioactive polysaccharose dressing for wound restoration as well as preparation method and application thereof
  • Bioactive polysaccharose dressing for wound restoration as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation of embodiment 1 Eucommia polysaccharide EUP3

[0045] Eucommia polysaccharide EUP3 was prepared by the following method: take 500 g of Eucommia bark (Kangmei Pharmaceutical, Guangzhou), crush it, add deionized water 10 times the mass of the raw material for extraction, and extract with stirring at a temperature of 90-100 °C for 3-4 hours, and filter and separate For the filter residue, add 4 times the volume of absolute ethanol (analytical grade, Sinopharm Group, Shanghai) to the filtrate, put it in a refrigerator at 4°C for overnight precipitation, and filter; remove the protein by savage method, dialyze, and freeze-dry to obtain the Eucommia polysaccharide rough sugar; use DEAE Ion exchange resin (Waterman, UK) and gel chromatographic column (Sigma, USA) were used to purify the Eucommia polysaccharide crude sugar obtained, and the distillate was collected and freeze-dried to obtain a homogeneous Eucommia polysaccharide EUP3.

Embodiment 2

[0046] Example 2 The effect of Eucommia polysaccharide EUP3 on enriching growth factors

[0047] Prepare polysaccharide solution (1 μg / mL), incubate with PDGF-BB growth factor at 37°C for 2 hours, observe with atomic force microscope, the results are as follows: figure 1 As shown, a single polysaccharide presents a thicker coiled and helical structure under the atomic force microscope; when the Eucommia polysaccharide is incubated with PDGF-BB to form a complex, it presents a thinner coiled coil structure, indicating that the surface of the sugar chain is bound to positively charged PDGF-BB. After BB, the electrostatic repulsion presents a more dispersed state.

Embodiment 3

[0048] The preparation of embodiment 3 bioactive polysaccharide dressings of the present invention

[0049] The bioactive polysaccharide dressing was prepared by electrospinning technology, using Eucommia polysaccharide EUP3 as the active material, gelatin (derived from pig skin, purchased from sigma company) as the carrier material; Fluoroethanol was used as a solvent to prepare gelatin solution (10%, w / w); polysaccharide solution and gelatin solution (1:9, v / v) were prepared respectively to prepare a solution suitable for spinning, and electrospinning was carried out under 12kv conditions. The distance between the receiving plates was set to 10 cm, the advancing speed of the syringe pump was 0.5 mL / h, and the spinning ambient temperature was 35 °C. Collect to obtain polysaccharide fiber dressing. Select glutaraldehyde vapor for cross-linking, the concentration is 1wt%, and the cross-linking time is 20min. Use a scanning tunneling microscope to observe, as shown in figure ...

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Abstract

The invention provides a bioactive polysaccharose dressing for wound restoration, particularly for chronic wound restoration as well as a preparation method and application thereof. The bioactive polysaccharose dressing is prepared by a technology of firstly preparing eucommia polysaccharide and carrier materials into a spinning solution and then performing static spinning. The preparation methodof the dressing provided by the invention is simple; the implementation is easy; the prepared biological dressing has good wound adhesion force; safety, non-toxicity and convenient application are realized. The bioactive polysaccharose dressing for wound restoration achieves the wound surface isolation function, has the good ventilation performance and simultaneously achieves the effects of stopping bleeding, preserving moisture, effectively enriching PDGF-BB and promoting chronic wound heeling. The dressing is particularly applicable to treatment and caring of clinically and widely existing chronic difficult-to-heal wound, particularly the diabetic ulcer.

Description

technical field [0001] The invention belongs to the field of novel polysaccharide wound dressings, and in particular relates to a bioactive polysaccharide dressing prepared by electrospinning for wound repair (especially chronic wound repair) and its preparation method and application. Background technique [0002] With the development of the economy and the continuous improvement of living standards, the diet structure of Chinese people has undergone tremendous changes. However, due to lack of exercise and other reasons, the number of diabetics in China has exploded in recent years. According to the World Health Organization, there were approximately 110 million diabetics in China in 2016, accounting for one-tenth of China's adult population. Diabetes patients are not only huge in number, but also face serious health threats. According to surveys, the number of deaths caused by non-communicable diseases such as diabetes accounts for 80% of the total number of deaths every...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/44A61L15/42A61K31/715A61P17/02A61P3/10D01D5/00
CPCA61K31/715A61L15/28A61L15/42A61L15/44A61L2300/232A61L2300/412A61L2400/04A61P3/10A61P17/02D01D5/0015C08L5/00
Inventor 王春明李秋张哲王一涛
Owner UNIVERSITY OF MACAU
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