Automatic analysis method of biodiversity

Abstract

The invention discloses an automatic analysis method of biodiversity. The method comprises the following specific analysis steps: step 1, using 24 pairs of primers containing sequences matched to an illumina sequencing chip, 8-base index sequences, sequencing primer sequences, difference sequences and universal amplification primer sequences, obtaining a library of on-machine sequencing through PCR reaction, and selecting a corresponding sequencing mode; step 2, converting stopping data into a fastq format by bcl2fastq software, and adding a parameter ''-create-fastq-for-index-reads''; step 3,using a Phred algorithm to intercept low-quality sequences through a filtering software BBDuk software package; and step 4, using a script in a QIIME package for secondary filtering on Tags sequencesobtained by splicing. According to the method, a project cycle is compressed, a database construction method, a data filtering method and software are improved, analysis deviations brought by chimeras and sequencing errors are effectively decreased, processes from data production to filtering and cleantags forming can be uniformity carried out without the need for distinguishing of each project even for microbial diversity projects, and processing time is saved.

Description

technical field [0001] The invention relates to the field of biological diversity analysis, in particular to an automatic analysis method for biological diversity. Background technique [0002] For the microbial diversity analysis project, the number of samples is large, the library construction period is long, the data volume of each sample is unbalanced, the filtering standards are different, and chimeras will appear during the polymerase chain reaction (PCR), which is easy to affect subsequent analysis. Moreover, as the amount of microbiological analysis data continues to increase and the standard analysis process is becoming more mature, it has become a challenge to analyze the standard process in an innovative, fast, efficient, and visualized way, and to conduct more in-depth personalized analysis. [0003] Therefore, it is necessary to invent an automatic analysis method of biodiversity to solve the above problems. Contents of the invention [0004] The purpose of t...

AI Technical Summary

Problems solved by technology

[0002] For the microbial diversity analysis project, the number of samples is large, the library construction period is long, the data volume of each sample is unbalanced, the filtering standards are different, and chimeras will appear during the polymerase chain reaction (PCR), which is easy to affect subsequent analysis. Moreover, as the amount of microbial analysis data continues to increase and the standard analysis process is becoming more mature, it has become a challenge to analyze the standard process in an innovative, fast, efficient, and visualized way, and to conduct in-depth personalized analysis.

Images