High-efficiency and high-sensitivity biomacromolecular membrane transferring and dyeing system and equipment

Abstract

The invention discloses a high-efficiency and high-sensitivity biomacromolecular membrane transferring and dyeing system, which comprises a work unit and an operation part, wherein the work unit is provided with at least one passage for detecting analysis samples; the operation part is associated with the work unit; the operation part creates at least one dyeing or membrane transferring passage parameter; each dyeing or membrane transferring passage parameter comprises at least one electric field circulation time. The invention also discloses equipment based on the high-efficiency and high-sensitivity biomacromolecular membrane transferring and dyeing system. The equipment comprises a base and a casing, wherein an operation panel is arranged outside the casing; a work region, a system power supply and a work circuit board are arranged in the base and the casing; the system power supply and the work circuit board are connected with the work region; the work region is at least provided with a pulling type independent work chamber; a positive and negative electrode board used for providing an electric field is arranged in the work chamber. The system or equipment capable of controlling the fast biomacromolecular membrane transferring and dyeing through the motion of bioelectric particles driven by the electric field is provided; meanwhile, the high-sensitivity requirement of the biological detection can also be ensured.

Description

[0001] This application is a divisional application with application number 201310025508.2, application date 2013.01.22, and application name "An Efficient and Highly Sensitive Biomacromolecule Transfer and Staining System and Equipment". technical field [0002] The invention relates to the technical field of biological detection equipment, in particular to a biomacromolecule transmembrane and dyeing system and equipment that utilizes an electric field to drive the movement of organisms or other charged particles to achieve high efficiency and high sensitivity. Background technique [0003] In a DC electric field, the phenomenon that charged particles move to electrodes with opposite signs is called electrophoresis (electrophoresis). In 1809, the Russian physicist Peнce first discovered the phenomenon of electrophoresis, but it was not until 1937 that Tiselius in Sweden established the boundary electrophoresis (boundary electrophoresis) for separating proteins that the elect...

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Problems solved by technology

Since the factors affecting the electrophoretic mobility are mainly related to the electric field strength, the pH value of the solution, and the ionic strength of the solution, the mobility of biomacromolecules and dyes in the medium where the electric field is applied is not the same, and biomacromolecules are larger. It moves slowly in the electric field and will not move out of the medium within a limited time. Organic dye molecules are much smaller than biomacromolecules, and dye molecules that are not combined with biomacromolecules can quickly move out of the medium, so they can be In the same electric field, the color development of biomacromolecules can be achieved by moving the dye out of the medium while the biomacromolecule (incorporated with the dye) remains in the medium, so that high resolution, little or even no background influence can be obtained in a short time. Analytical images of biomacromolecules in the background, but if the dye and biomacromolecules only meet and combine once, it is difficult to meet the requirements for some experiments with relatively high sensitivity requirements, and even prone to missed detection in the detection

[0005] With the rapid development of biomedicine and other disciplines, the detection technology of biomacromolecules is also rapidly improving. However, there is no system or device in the market that can use electric fields to drive the movement of biologically charged particles to control the rapid dyeing and transfection of biomacromolecules. membrane, and at the same time achieve the requirement of high sensitivity

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