Loop-mediated isothermal amplification fluorescence detection method, primer and kit for identifying radix notoginseng ingredient, and application

Abstract

The invention relates to a loop-mediated isothermal amplification fluorescence detection method, primer and kit for identifying the radix notoginseng ingredient, and application, and belongs to the technical field of biological detection. The novel technology of fusing nucleic acid in vitro rapid amplification and fluorescence detection technologies is used; six specific primers are designed by aiming at target fragments; the whole isothermal amplification nucleic acid replication reaction process is monitored in real time through an amplification curve; the product is subjected to solubilitycurve analysis. A loop-mediated isothermal amplification method for identifying the radix notoginseng ingredient is built; the DNA level fast qualitative detection on whether the radix notoginseng iscontained in a sample to be tested or not is performed; the detection use time is greatly shortened; the fast detection of the radix notoginseng ingredient is realized; the computer experiment can beperformed without purification after DNA extraction; the six primers correspond to the recognition of eight specific regions of a target sequence, so that high-degree specificity of the detection reaction is ensured; good application prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a ring-mediated isothermal amplification fluorescence detection method, primers, kit and application for identifying components of Panax notoginseng. Background technique [0002] Panax notoginseng is a traditional precious Chinese medicinal material in my country, one of the 40 kinds of bulk medicinal materials. It has a history of more than 400 years of application and has a long-standing reputation at home and abroad. Sanqi has been known as the "hemostatic medicine" since ancient times. Due to the unique and obvious effects of Panax notoginseng, more and more people began to take it in their daily life; there are more than 360 kinds of Chinese patent medicines using it as raw materials, involving more than 1,350 pharmaceutical companies; its price remains high, and price fluctuations are enough to affect the Thousands of pharmaceutical companies. Wha...

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Problems solved by technology

[0003] Because Panax notoginseng is expensive, in order to make huge profits, many lawbreakers confuse real ones with inferior ones, causing inconvenience to customers who buy Panax notoginseng. , are common targets for adulteration, and the processed Sanqi powder is easier to manipulate

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