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Extraction method of coptis alkaloids

A Coptis alkaloid and extraction technology, which is applied in the directions of pharmaceutical formulations, plant raw materials, and medical preparations containing active ingredients, can solve the problems of low extraction rate, long extraction time, and complicated extraction method operations, and achieves simple operation, acetonitrile, etc. less toxic effect

Active Publication Date: 2021-04-02
长沙高新开发区岳麓山大学科技园科技服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to provide a method for extracting Coptidis alkaloids, so as to solve the technical problems of complicated operation, long extraction time and low extraction rate of existing Coptidis alkaloids extraction methods

Method used

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  • Extraction method of coptis alkaloids
  • Extraction method of coptis alkaloids
  • Extraction method of coptis alkaloids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Grind 0.1g Coptis rhizoma into powder, add 25mL of 1mol / L hydrochloric acid solution, heat and reflux at 80°C for extraction for 1 hour, filter and collect the filtrate to obtain filtrate and filter residue, add 25mL of 1mol / L hydrochloric acid solution to the filter residue again In the method, extract by heating under reflux at 80° C. for 1 h, filter and collect the filtrate, mix the obtained filtrates, and freeze-dry to obtain the crude extract of Coptis rhizome.

[0045] Mix acetonitrile and water at a volume ratio of 6:4 to prepare an aqueous acetonitrile solution, add 10 mg of Coptis rhizome crude extract to 55 ml of acetonitrile aqueous solution, and vortex for 1 min to obtain a mixed solution.

[0046] Add 1.0 g of ammonium sulfate to the mixed solution, vortex, centrifuge and stand for stratification, the upper phase is the acetonitrile phase, the lower phase is the water phase, the alkaloid of Coptidis Rhizoma is dissolved in the acetonitrile phase, and the upp...

Embodiment 2

[0048] Grind 1kg of Coptidis rhizoma into powder, add it to 200L hydrochloric acid solution with a concentration of 1.2mol / L, extract it with a multifunctional extraction tank at 100°C for 1 hour, filter and collect the filtrate to obtain the first filtrate and filter residue, and add the filter residue again to 200L with a concentration of In a 1.2mol / L hydrochloric acid solution, heat and reflux at 100°C for 1 hour, filter and collect to obtain the second filtrate, mix the first filtrate and the second filtrate, and freeze-dry to obtain the crude extract of Coptis rhizome .

[0049] Mix acetonitrile and water at a volume ratio of 8:2 to prepare an aqueous acetonitrile solution, add 15 g of Coptis rhizome crude extract to 100 L of acetonitrile aqueous solution, and vortex for 2 minutes to obtain a mixed solution.

[0050] Add 2.2kg of ammonium sulfate to the mixed solution, vortex, centrifuge and leave to separate layers, the upper phase is the acetonitrile phase, the lower p...

Embodiment 3

[0052] Grind 3g of Coptis Rhizoma into powder, add 1L of hydrochloric acid solution with a concentration of 0.8mol / L, heat and reflux at 60°C for extraction for 1 hour, filter and collect the filtrate to obtain the first filtrate and filter residue, and add 1L of the filter residue again with a concentration of 0.8mol / L hydrochloric acid solution, heated and refluxed at 60°C for 1 h, filtered and collected to obtain the second filtrate, mixed the first filtrate and the second filtrate, and freeze-dried to obtain the crude extract of Coptis rhizome.

[0053] Mix acetonitrile and water at a volume ratio of 6:4 to prepare an aqueous acetonitrile solution, add 300 mg of Coptis rhizome crude extract to 0.6 L of acetonitrile aqueous solution, and vortex for 1.5 min to obtain a mixed solution.

[0054] Add 1.5g of ammonium sulfate to the mixed solution, vortex, centrifuge and let stand to separate layers, the upper phase is the acetonitrile phase, the lower phase is the water phase, ...

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Abstract

The invention discloses an extraction method of alkaloid of rhizoma coptidis. The extraction method comprises the following steps: grinding the rhizoma coptidis into powder, adding the rhizoma coptidis powder to a hydrochloric acid solution, implementing crude extraction, filtering and collecting a filtrate, and freeze-drying the collected filtrate, so that a crude extract of rhizoma coptidis is obtained; mixing acetonitrile and water at the volume ratio of (2-12) to 3, so that an acetonitrile water solution is prepared, then adding the prepared crude extract of rhizoma coptidis to the acetonitrile water solution, and implementing vortex oscillation, so that a mixed solution is obtained; and adding ammonium sulfate to the prepared mixed solution, implementing vortex oscillation and uniformly mixing all materials, conducting centrifuging, standing by and layering, reserving an upper phase and implementing drying, so that the alkaloid of rhizoma coptidis is obtained. The extraction method of the alkaloid of rhizoma coptidis provided by the invention is high in extraction efficiency and simple in extraction method.

Description

technical field [0001] The invention relates to the technical field of extraction of plant components, in particular to an extraction method of coptis alkaloids. Background technique [0002] Coptis chinensis is a commonly used Chinese herbal medicine, which has the effects of clearing away heat and dampness, purging fire and detoxifying. Its main active ingredients are alkaloids of Coptis chinensis, including berberine, palmatine, jatrorrhizine and coptisine, among which berberine The highest content. Research has found that the alkaloids in Coptidis root have anti-inflammatory, antibacterial, hypoglycemic, hypotensive, anti-ulcer, anti-tumor, and neuromodulatory effects. The traditional methods of extracting alkaloids from Coptis chinensis mostly use organic solvent extraction, and then purify them by recrystallization and other means. The extraction steps are many and require the use of a large amount of organic solvents, which easily leads to problems such as solvent re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/718
CPCA61K36/718A61K2236/331A61K2236/333A61K2236/53
Inventor 马铭王莹陈波李扶桑赵文翔
Owner 长沙高新开发区岳麓山大学科技园科技服务有限公司
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