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Method for extracting low-molecular-weight peptide from dairy products

A technology for low-molecular-weight peptides and dairy products, applied in peptide preparation methods, chemical instruments and methods, peptides, etc., can solve the problems that low-molecular-weight peptides have a bitter taste and are not easily accepted by consumers, and achieve functional enhancement and simple and feasible extraction methods , good application value and market potential

Inactive Publication Date: 2018-07-31
金华市铁骑士生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the low-molecular-weight peptide obtained by this preparation method has a bitter taste and is not easily accepted by consumers.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for extracting low-molecular-weight peptides from dairy products, comprising compound enzyme hydrolysis, ultrafiltration separation, and macroporous resin desalination, the specific steps of which are:

[0016] 1) Compound enzyme hydrolysis: add distilled water to the skim milk product according to the material-to-liquid ratio of 1:25 (g / mL), mix well, adjust the pH to 8.0, and add the compound enzyme according to the amount of enzyme added at 6% (w / w) , then add tyrosine, mix evenly, enzymatically hydrolyze at 45°C for 6h, then place in boiling water to inactivate the enzyme for 8min, and then centrifuge at 6000r / min for 15min, the supernatant is the enzymatic hydrolyzate, the above The compound enzyme is a mixture of alkaline protease, Bacillus licheniformis protease and flavor protease, and its weight ratio is 1:1.3:0.3. The composition and activity of molecular peptides will also be different, and the effect is better than that of single-enzyme hydrolysis. ...

Embodiment 2

[0021] A method for extracting low-molecular-weight peptides from dairy products, comprising compound enzyme hydrolysis, ultrafiltration separation, and macroporous resin desalination, the specific steps of which are:

[0022] 1) Compound enzyme hydrolysis: Add distilled water to the skim milk product at a material-to-liquid ratio of 1:15 (g / mL), mix well, adjust the pH to 9.0, and add compound enzyme at an enzyme amount of 4% (w / w) , then add tyrosine, mix evenly, enzymatically hydrolyze at 55°C for 4 hours, then place in boiling water to inactivate the enzyme for 15min, and then centrifuge at 4000r / min for 25min, the supernatant is the enzymatic hydrolyzate, the above The compound enzyme is a mixture of alkaline protease, Bacillus licheniformis protease and flavor protease, and its weight ratio is 1:1.1:0.5;

[0023] 2) Ultrafiltration separation: The enzymatic hydrolysis solution is subjected to ultrafiltration with 8kDa, 6kDa and 1kDa molecular weight cut-off ultrafiltrati...

Embodiment 3

[0027] A method for extracting low-molecular-weight peptides from dairy products, comprising compound enzyme hydrolysis, ultrafiltration separation, and macroporous resin desalination, the specific steps of which are:

[0028] 1) Compound enzyme hydrolysis: add distilled water to the skim milk product according to the material-to-liquid ratio of 1:20 (g / mL), mix well, adjust the pH to 8.5, and add compound enzyme according to the enzyme amount of 5% (w / w) , then add tyrosine, mix evenly, enzymatically hydrolyze at 50°C for 5 hours, then place in boiling water to inactivate the enzyme for 12min, and then centrifuge at 5000r / min for 20min, the supernatant is the enzymatic hydrolyzate, the above The compound enzyme is a mixture of alkaline protease, Bacillus licheniformis protease and flavor protease, and its weight ratio is 1:1.2:0.4;

[0029] 2) Ultrafiltration separation: The enzymatic hydrolysis solution is subjected to ultrafiltration with ultrafiltration membranes with mole...

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Abstract

The invention discloses a method for extracting low-molecular-weight peptide from dairy products. The method comprises the specific steps of adding distilled water, compound enzymes and tyrosine intothe skimmed dairy products, conducting enzymolysis and enzyme deactivation and centrifuging liquid supernatant to obtain enzymatic hydrolysate; conducting ultrafiltration on enzymatic hydrolysate through ultrafiltration membranes in sequence and freeze-drying filtrate; preparing a solution from a frozen sample subjected to ultrafiltration through double distilled water, adding the solution into acolumn, eluting the solution through an ethyl alcohol solution and freezing and drying eluent to obtain low-molecular-weight peptide. The method has the advantages that low-molecular-weight activatedcarbon is simple and feasible in extracting method and high in extracting efficiency, yield and purity, the biological activity of low-molecular-weight peptide is high, and low-molecular-weight peptide is free of bitter taste, has the fragrance of the dairy products, high pH stability and digestion resistance, is not easily further subjected to enzymolysis by digestive enzymes and can be directlyused by an organism.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a method for extracting low-molecular-weight peptides from dairy products. Background technique [0002] The low-molecular-weight peptide derived from protein is a multifunctional factor, which consists of small peptides with short peptide chains of several amino acid residues, oligopeptides (5-10 amino acids) and polypeptides (11-50 amino acids); this Peptoids can be directly absorbed and utilized by the body, and have the characteristics of high absorption efficiency and low immunogenicity. In addition, they also play an important role in the regulation of cell physiology and metabolic functions, which involve almost all physiological activities of the human body, such as the nervous system, digestive system, circulatory system, and endocrine system. Not only that, but many of these peptides have new functions not found in the original protein or its constituent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16
CPCC07K1/16C07K1/34C07K1/36C12P21/06
Inventor 全盈园
Owner 金华市铁骑士生物科技有限公司
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