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Method for realizing distant hybridization of vigna angularis and vigna umbellata by utilizing vigna riukiuensis as bridge parent

A bridge parent and distant hybridization technology, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve problems such as genetic barriers, excellent traits of transgenic rice beans, and embryo degeneration, so as to avoid embryo abortion, Guarantee the effect of hybridization pod yield

Pending Publication Date: 2018-07-31
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although direct crosses between adzuki bean and rice bean can set pods, due to genetic barriers, embryogenesis degenerates in the later stages of development and cannot produce viable seeds
Therefore, the excellent traits of rice bean cannot be bred by direct crossing between adzuki bean and rice bean

Method used

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  • Method for realizing distant hybridization of vigna angularis and vigna umbellata by utilizing vigna riukiuensis as bridge parent
  • Method for realizing distant hybridization of vigna angularis and vigna umbellata by utilizing vigna riukiuensis as bridge parent
  • Method for realizing distant hybridization of vigna angularis and vigna umbellata by utilizing vigna riukiuensis as bridge parent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Adzuki bean and Ryukyu cowpea (Vigna riukiuensis) hybridization to obtain bridge offspring

[0030] 1. Test materials

[0031] The adzuki bean variety Jihong 352 bred by the Institute of Grain and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences was selected as the female parent, and the adzuki bean close wild species V.riukiuensis-2 was used as the first male parent, that is, the bridge parent.

[0032] Wherein, the adzuki bean variety Jihong 352 and the adzuki bean close relative wild species V.riukiuensis-2 are preserved and provided by the Grain and Oil Crops Research Institute of Hebei Academy of Agriculture and Forestry Sciences.

[0033] 2. Test method

[0034] Material planting: sow the parent materials in flower pots, among which the first male material V. riukiuensis-2 is planted in 5 pots, and one seedling is reserved in each pot. At the seedling stage, short-day treatment was adopted, with 13 hours of dark treatment every day, and co...

Embodiment 2

[0039] Hybridization between embodiment 2, bridge progeny and rice bean

[0040] 1. Test materials

[0041]The bridge offspring obtained in Example 1 was selected as the female parent, and the rice bean material F021 with better comprehensive properties was used as the male parent.

[0042] Among them, the rice bean material F021 with better comprehensive properties is preserved and provided by the Grain and Oil Crops Research Institute of Hebei Academy of Agriculture and Forestry Sciences.

[0043] 2. Test method

[0044] Material planting: Sow the hybrid parent materials in flower pots, and plant 5 pots of the female parent material bridge offspring, and keep 1 seedling in each pot. The male parent material Fandou F021 was sown in three batches at the wrong time, the first batch was sown 7 days earlier than the offspring of Qiaoqiao, the second batch was sown at the same time as the offspring of Qiaoqiao, and the third batch was sown 7 days after the staggered time to ensu...

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Abstract

The invention discloses a method for realizing distant hybridization of vigna angularis and vigna umbellata by utilizing vigna riukiuensis as a bridge parent. The method provided by the invention selects a vigna angularis variety with excellent comprehensive traits as a female parent, selects the vigna angularis wild relative vigna riukiuensis as a first male parent, that is, the bridge parent, and selects a vigna umbellate material with excellent comprehensive traits as a second male parent; and firstly, the vigna riukiuensis and the vigna angularis are subjected to hybridization to obtain abridge progeny, and then the bridge progeny and the vigna umbellate are subjected to hybridization, through the method, the difficult problem of embryo abortion when the vigna angularis and the vignaumbellate are directly subjected to distant hybridization can be effectively avoided, the obtained distant hybrid grains have vitality, seedlings can be emerged normally, and flowering and fructification can be performed, so that interspecific distant hybridization of the vigna angularis and the vigna umbellate is realized.

Description

technical field [0001] The invention relates to a method for distant hybridization of adzuki bean and rice bean, in particular to a method for realizing distant hybridization of adzuki bean and rice bean by using Ryukyu cowpea as a bridge parent. The invention belongs to the field of biotechnology breeding. Background technique [0002] Adzuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] is native to China, and is planted in all parts of my country except for a few alpine regions. The main production areas are in North China, Northeast China and the Jianghuai region. my country is the world's largest producer of small soybeans, and its total output and export volume both rank first in the world. Cultivated rice bean [Vigna umbellata (Thunb.) Ohwi & Ohashi] and adzuki bean belong to Vigna genus (Vigna) Asian cowpea subgenus (Ceratotropis), it has the advantages of heat resistance, drought resistance, acid soil resistance, etc., and most cultivated rice bean varieties are Be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 刘长友田静范保杰曹志敏关中波苏秋竹张志肖王彦
Owner INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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