A method for distant hybridization of Malvaceae crops

Abstract

The invention relates to the field of agriculture, in particular to a method for distant hybridization of Malvaceae crops. The method for the distant hybridization of the Malvaceae crops is as follows: using the upland cotton seedlings as rootstocks and the goldenflower seedlings as scions for grafting to obtain grafted seedlings; when the grafted seedlings bloom, all the flower buds of the grafted seedlings are Remove the stamens, apply the pollen of upland cotton on the bud stigma of the grafted seedlings, inject the nutrient solution into the base of the pistil of the grafted seedlings; obtain and collect the seeds of hybrid bolls; the nutrient solution includes: 0.1-2 Brassinolide; 0.1 to 2 diethyl diethyl ethyl; 0.1 to 3 indole-3-acetic acid; 0.1 to 2 naphthalene acetic acid; 0.1 to 2 forchlorfenuron; 10 to 50 parts of C2 to C5 Alcohol; 50000-120000 parts of water. The method obtains distant hybrid seeds and hybrid plants of upland cotton and goldenflower, improves the seed setting rate of hybrid young bolls, and reduces the shedding rate of young bolls.

Description

technical field [0001] The invention relates to the field of agriculture, in particular to a method for distant hybridization of Malvaceae crops. Background technique [0002] Upland cotton, an annual herb belonging to Malvaceae, is an important national strategic material. Cotton bollworm once occurred in large areas in major cotton regions in my country, and the planting of Bt insect-resistant cotton has basically controlled the occurrence of cotton bollworm. However, secondary pests such as aphids and red spiders have gradually become the main pests that harm cotton growth, seriously affecting cotton yield and quality. At present, production mainly relies on pesticide control, which increases the cost of cotton planting on the one hand, and easily causes environmental pollution on the other hand. Therefore, cultivating cotton varieties resistant to aphids and red spiders, and using the resistance of the crops to prevent and control aphids and red spiders has always been...

AI Technical Summary

Problems solved by technology

[0004] However, there are technical and genetic barriers in distant hybridization. After the hybridization of upland cotton and sunflower, the young sunflower bolls can usually grow and develop normally, and begin to fall off after 3 days, and reach the peak of shedding in 5-7 days , only about 3% of hybrid bolls can develop into normal bolls

However, even if the hybrid bolls appear to be normally developed, they are peeled off after the fruit is ripe, see figure 1 , rows of aborted immature embryos can be found without normal hybrid seed production

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