A method of realizing distant hybridization of adzuki bean and rice bean by using immature embryo rescue

Abstract

The invention discloses a method for realizing distant hybridization of adzuki beans and rice beans by utilizing young embryo rescue. The method comprises the steps of (1) preparation of materials, namely, selecting more early-matured rice bean material as female parent, selecting adzuki bean variety with excellent comprehensive characters as male parent, and selecting disease-free full grains for planting; (2) material planting; (3) cross pollination; (4) young embryo rescue; (5) hybrid seedling transplanting. According to the method, the more early-matured rice bean material is taken as the female parent, and the adzuki bean variety with excellent comprehensive characters is taken as the male parent, so that the obtained hybrid young embryos can directly grow into seedlings, the seedlings are good in growth and development and high in survival rate of transplanting, distant hybridization F1 plants can normally bloom and bear fruit at the adult-plant stage, and seeds of F2 can be harvested. After the method is used, a great deal of distant hybridization embryos can continue to develop into normal seeds, abortion of distant hybrid embryos can be effectively avoided, a great deal of distant hybridization filial generations can be obtained, and interspecific distant hybridization of the adzuki beans and the rice beans can be finally realized.

Description

technical field [0001] The invention relates to a method for distant hybridization of adzuki bean and rice bean, in particular to a method for realizing distant hybridization of adzuki bean and rice bean by using immature embryo rescue, and the invention belongs to the field of biotechnology breeding. Background technique [0002] Adzuki bean [Vignaangularis (Willd.) Ohwi&Ohashi] is native to China, except for a few alpine regions, it is planted all over my country, and its main production areas are in North China, Northeast China and Jianghuai region. my country is the world's largest producer of small soybeans, and its total output and export volume both rank first in the world. Bean weevil is a kind of storage pest in the production process of adzuki bean. The weight of adzuki bean harmed by bean weevil is reduced, the nutrition is lost, and the quality is seriously reduced. The main method for preventing and controlling soybean weeds in production is aluminum phosphide ...

AI Technical Summary

Problems solved by technology

However, adzuki bean is a small crop in my country, and its breeding and genetic research is relatively backward. The current main breeding methods are still based on natural variation selection and intraspecific hybridization, and there is a lack of research reports on distant hybridization.

There is no domestic research report on the distant hybridization of Adzuki bean and Rice bean

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