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Magnetic sensor based on longitudinal relaxation time signal readout, method for constructing same and use of same

A longitudinal relaxation time and magnetic sensor technology, applied in instruments, scientific instruments, measuring magnetic variables, etc., can solve the problems of false positive signals, high cost, and the magnetic relaxation time sensor is not suitable for biochemical analysis, etc., to improve the analysis efficiency , The effect of widening the limit of the detection range

Active Publication Date: 2018-07-27
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all require the preparation of "nanomagnetic particle-antibody" conjugates, which will waste a lot of antibodies, and the operation is cumbersome.
At the same time, this method can only take advantage of the high specificity of the immune response and is only suitable for immune analysis
If it is used for biochemical analysis, its corresponding antibody is required, resulting in complex analysis and high cost, so traditional magnetic relaxation time sensors are not suitable for biochemical analysis
Traditional magnetic relaxation time sensors use nano-magnetic particles as signal probes, but the magnetic probe has the following two problems: (1) The specific surface area of ​​nano-magnetic particles is large, which is easy to cause non-specific adsorption, so it is easy to cause false positives. Positive; (2) Nanomagnetic particles are easy to self-aggregate in complex biological or food samples, resulting in false positive signals

Method used

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  • Magnetic sensor based on longitudinal relaxation time signal readout, method for constructing same and use of same
  • Magnetic sensor based on longitudinal relaxation time signal readout, method for constructing same and use of same
  • Magnetic sensor based on longitudinal relaxation time signal readout, method for constructing same and use of same

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Alkaline phosphatase (ALP) is an important marker enzyme in immunoassay, and alkaline phosphatase can catalyze its substrate (ascorbate) to produce reductive vitamin C (V C ), the V C The mediated redox reaction can realize the Fe 2+ and Fe 3+ The conversion between the two ions, on the one hand, realizes the detection of ALP, because ALP itself is also an important biomarker. At the same time, it is one of the most widely used immunolabeling enzymes, and the T-based 1 The magnetic sensor of the signal can realize the combination of immunoassay and biochemical analysis.

[0047] In terms of immunoassays, alkaline phosphatase (ALP) can remove a phosphate group from ascorbic acid ester through an enzymatic reaction to become ascorbic acid (V C ), V C Fe can be 3+ become Fe 2+ , thus realizing the T-based 1 Immunoassay of signal, through immunoassay, detection of various targets can be realized.

[0048] T 1 Magnetic sensor detects vitamin C (V C )

[0049] ...

Embodiment 2

[0053] The construction of a magnetic sensor based on longitudinal relaxation time signal readout can detect glucose in blood, and glucose produces hydrogen peroxide (H2O2) under the action of glucose oxidase (GOD). 2 o 2 ), H 2 o 2 Oxidizing, Fe 2+ Convert to Fe 3+ , resulting in T 1 signal changes through T 1 The signal indirectly reflects the content of the target substance (glucose). That is to say, many kinds of biochemical analysis indicators can be detected through this redox. Or use glucose oxidase (GOD) as a marker enzyme in immunoassays to generate H 2 o 2 , H 2 o 2 Through redox reactions, Fe can 3+ / Fe 2+ transition, which in turn causes the magnetic signal T 1 The change of the signal finally converts the content signal of the target substance in the sample to T 1 signal change.

[0054] T 1 Sensors for different concentrations of H 2 o 2 The response result of

[0055] 20 μL of H 2 o 2 The solution was added to a 96-well microwell, and th...

Embodiment 3

[0059] The detection of alpha-fetoprotein (AFP) in serum is realized through immunoassay reaction.

[0060] Experimental steps:

[0061] (1) Dilute the capture antibody that recognizes AFP to 5 μg / mL with coating solution (carbonate buffer, pH 9.6), and add to the wells of the ELISA plate, 100 μL / well. Placed at 37°C, reacted for 2h.

[0062] (2) At room temperature, shake off the solution in the well and pat dry. Add PBS solution containing 0.5‰ (volume) Tween-20, 150 μL / well, let stand for 1 min, shake off the washing solution in the well, and pat dry. Repeat the wash 3 times.

[0063] (3) Blocking: add 100 μL / well of 3% BSA, place at 37° C. for 2 hours.

[0064] (4) At room temperature, shake off the solution in the well and pat dry. Add PBS containing 0.5‰ (volume) Tween-20, 150 μL / well, shake off the washing solution in the well, pat dry, repeat washing 3 times, store at -20°C until use.

[0065] (5) Dilute AFP (1000ng / mL) 2-fold with PBS to 7.8125ng / mL, add 100μL / w...

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Abstract

The invention provides a magnetic sensor based on longitudinal relaxation time signal readout. The magnetic sensor takes a longitudinal relaxation time (T1) as a readout signal. The invention also provides a method for constructing a magnetic sensor based on longitudinal relaxation time signal readout, and the use of the magnetic sensor in the fields of biochemical analysis and immunoassay, food safety, environmental detection and the like.

Description

technical field [0001] The invention belongs to the technical field of biomolecular recognition. Specifically, the invention relates to a magnetic sensor based on longitudinal relaxation time signal readout and its construction method and application. Background technique [0002] Magnetic relaxation time sensors, as a new type of magnetic sensor technology, have been widely used in clinical diagnosis, environmental monitoring, food safety and other fields. The biggest advantage of magnetic sensing analysis is that because the magnetic signals of biological samples and environmental samples are basically negligible, this method does not require complicated sample pretreatment steps. At present, the magnetic relaxation time sensors mainly include: (1) based on The transverse relaxation time (T 2 ) immunosensor; (2) magnetic relaxation time immunosensor combining magnetic separation and magnetic relaxation time: based on the significant difference in the separation speed of s...

Claims

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Application Information

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IPC IPC(8): G01R33/00G01N27/72G01N27/74G01N33/50G01N33/53G01N33/573
CPCG01N27/72G01N27/74G01N33/50G01N33/53G01N33/573G01R33/0023
Inventor 蒋兴宇陈翊平
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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