Goat whey protein immunoregulation peptide as well as preparation method and application thereof
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A technology of immune regulation and goat whey, which is applied in the food field, can solve the problems of few reports on the immune regulation peptide sequence derived from goat milk, and achieve good immune regulation activity, high food safety, and good absorption effect
Active Publication Date: 2018-07-24
HYPROCA NUTRITION CO LTD
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However, there are few reports on the immuno...
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Embodiment 1
[0025] A goat milk protein immunomodulatory peptide, which includes two peptide sequences: Thr-Gly-Pro-Ile-Pro-Asn-Ser-Leu (TGPIPNSL) and Ala-Pro-Glu-Pro-Glu-Val-Phe (APEPEVF ).
[0026] The preparation method of the above-mentioned goat milk protein immunomodulatory peptide is as follows:
[0027] The first step is to weigh 10g of goat whey protein, dissolve it in 200mL of water, adjust the pH to 2.0 in a water bath at 50°C, add 40KU of pepsin, and then hydrolyze for 1 hour while maintaining the temperature and pH value to obtain the hydrolysis reaction system;
[0028] The second step is to keep the temperature of the hydrolysis reaction system constant, adjust the pH value of the hydrolysis reaction system to 8.0, add trypsin 50KU, and continue hydrolysis for 2 hours; raise the temperature of the reaction system to 85°C and keep it for 15 minutes to obtain goat milk protein hydrolyzate;
[0029] The third step is to cool the goat milk protein hydrolyzate to room temperatu...
Embodiment 2
[0032] A goat milk protein immunomodulatory peptide, which includes three peptide sequences: Thr-Gly-Pro-Ile-Pro-Asn-Ser-Leu (TGPIPNSL), Arg-Phe-Phe-Asp-Asp-Lys-Ile-Ala ( RFFDDKIA) and Ala-Pro-Glu-Pro-Glu-Val-Phe (APEPEVF).
[0033] The preparation method of the above-mentioned goat milk protein immunomodulatory peptide is as follows:
[0034] The first step is to weigh 15g of goat whey protein, dissolve it in 300mL of water, adjust the pH to 3.0 in a 55°C water bath, add 50KU of pepsin, and then hydrolyze for 2 hours while maintaining the temperature and pH value to obtain the hydrolysis reaction system;
[0035] The second step is to keep the temperature of the hydrolysis reaction system constant, adjust the pH value of the hydrolysis reaction system to 8.0, add trypsin 40KU, and continue hydrolysis for 2 hours; raise the temperature of the reaction system to 85°C and keep it for 20 minutes to obtain goat milk protein hydrolyzate;
[0036] The third step is to cool the goa...
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Abstract
The invention provides goat whey protein immunoregulation peptide. The goat whey protein immunoregulation peptide contains at least one peptide sequence of Thr-Gly-Pro-Ile-Pro-Asn-Ser-Leu, Arg-Phe-Phe-Asp-Asp-Lys-Ile-Ala and Ala-Pro-Glu-Pro-Glu-Val-Phe. The goat whey protein immunoregulation peptide is obtained from goat whey protein, and the raw materials are easily available; the goat whey protein immunoregulation peptide has very obvious promotion effect on the proliferation activity of spleen lymphocyte, shows certain dose dependent effect and has immunoregulation effect. The invention also discloses a preparation method of the goat whey protein immunoregulation peptide. The preparation method has simple steps; two kinds of protease (pepsase and trypase) are combined to hydrolyze the goat whey protein, so that active peptide fragments can be released well; the goat whey protein hydrolysate is separated and purified by a gel chromatographic column and high-performance liquid chromatography, and the purity of the product is higher. The invention also provides application of the goat whey protein immunoregulation peptide. The goat whey protein immunoregulation peptide is applied to preparation of food or health-care food with immunoregulation activity, and has wide application prospect.
Description
technical field [0001] The invention relates to the field of food technology, in particular to a goat whey protein immunomodulatory peptide, a preparation method and application thereof. Background technique [0002] At present, with the development of society, problems such as environmental pollution, social pressure, and population aging are becoming more and more serious. Under the influence of these factors, the body will inevitably experience a decline in immunity, which will lead to a series of health problems. In recent years, milk-derived immune active peptides have attracted much attention. Milk-derived immunoactive peptides are short peptides separated from milk proteins by protease hydrolysis, and are the second type of biologically active peptides found in milk proteins after morphine peptides. In previous reports, multiple immunomodulatory peptide sequences have been isolated from milk, such as TTMPLY, RYLGYLE, RYLGYL, FFVAPFPEVFGK derived from milk αs1-casein...
Claims
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