Porous silk fibroin spinal cord scaffold with directional release function, its preparation method, preparation mold and application

A technology of porous silk and spinal cord, applied in the field of spinal cord stent, can solve the problems of complex and time-consuming directional freezing operation

Active Publication Date: 2021-07-06
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, directional freezing has disadvantages such as complicated operation and time-consuming

Method used

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  • Porous silk fibroin spinal cord scaffold with directional release function, its preparation method, preparation mold and application
  • Porous silk fibroin spinal cord scaffold with directional release function, its preparation method, preparation mold and application
  • Porous silk fibroin spinal cord scaffold with directional release function, its preparation method, preparation mold and application

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0039] 1. Preparation of silk fibroin aqueous solution

[0040] Take the silkworm cocoon from which the chrysalis has been removed, and add 0.02mol / L Na 2 CO 3 Water solution, boiled for 30 minutes, took out, wrung out, rinsed with distilled water; repeated the above operation twice, dried in a 60°C oven for 24 hours, and kept loosening in the middle to obtain degummed silk; put the degummed silk in 9.3mol / L LiBr aqueous solution , the mass ratio of degummed silk to 9.3mol / L LiBr aqueous solution is 1:5, keep warm at 60°C until the degummed silk is completely dissolved, put it into a dialysis bag with a cut-off molecular weight of 8,000-14,000 Daltons, and dialyze with tap water at 4°C for two days, Dialyze with deionized water at 4°C for one day (change the water every 1 hour on the first day, and every 2 hours on the next two days, and the interval between water changes at night should not be too long). After dialysis, the solution is filtered through 8 layers of gauze and ...

Embodiment 1

[0046] In the step 1 of preparing the scaffold in Example 1, the total concentration of the mixed solution is 5%, and 20ml of the mixed solution of SF / HA=95:5 is added, and then EDC, 10% NHS, 10% NHS, 0.012g MES (the content of EDC, NHS, MES is relative to the content of SF in the SF / HA mixed solution), fully stirred for 1h under ice bath. Inject into the mold with a syringe (the number of needles is 10), and freeze in a refrigerator at -80°C for 24 hours. The above-mentioned frozen samples were taken out from the mold, placed in a freeze dryer, and dried under reduced pressure for 48 hours to obtain a multi-channel SF / HA porous scaffold.

Embodiment 2

[0048] In the step 1 of preparing the scaffold in Example 1, the total concentration of the mixed solution is 5%, and 20ml of the mixed solution of SF / HA=90:10 is added, and then EDC, 10% NHS, 10% NHS, 0.012g MES (the content of EDC, NHS, MES is relative to the content of SF in the SF / HA mixed solution), fully stirred for 1h under ice bath. Inject into the mold with a syringe (the number of needles is 10), and freeze in a refrigerator at -80°C for 24 hours. The above-mentioned frozen samples were taken out from the mold, placed in a freeze dryer, and dried under reduced pressure for 48 hours to obtain a multi-channel SF / HA porous scaffold.

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Abstract

The invention belongs to the technical field of spinal cord support, and in particular relates to a porous silk fibroin spinal cord support with directional release function, a preparation method, a preparation mold and application thereof. The method comprises steps: 1) preparing an aqueous silk fibroin solution; preparing a hyaluronic acid solution; 2) mixing the silk fibroin aqueous solution and the hyaluronic acid solution, and then adding EDC, NHS and MES in sequence to perform a cross-linking reaction in an ice bath, Thoroughly stir, vacuumize and defoam, then inject the reaction solution into the preparation mold, and then freeze the mold below -80°C for molding, remove the silicone tube of the mold after molding to obtain a sample, and then freeze-dry the sample. The porous silk fibroin spinal cord scaffold with directional release function was obtained after freeze-drying. The invention uses acupuncture needles to prepare channels, which can make the channels evenly and directionally distributed, and obtain a silk fibroin / hyaluronic acid composite porous scaffold with uniform material pore size, adjustable pore size, high porosity and good pore connectivity.

Description

technical field [0001] The invention belongs to the technical field of spinal cord support, and in particular relates to a porous silk fibroin spinal cord support with directional release function, a preparation method, a preparation mold and application thereof. Background technique [0002] An ideal scaffold should have the characteristics of suitable pore size, high porosity, good pore connectivity, high mechanical strength, and good biocompatibility. Research at home and abroad has initially proved that silk fibroin (SF) has certain advantages in repairing nerve tissue defects, and hyaluronic acid (HA) shows a variety of important physiological functions in the body due to its unique molecular structure and physical and chemical properties. . Silk fibroin is a protein extracted from silkworm cocoons, which has the advantages of high mechanical strength, good toughness, and high elasticity. Therefore, combining silk fibroin and nano-hyaluronic acid can overcome the defe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/50A61L27/54A61L27/56
CPCA61L27/26A61L27/50A61L27/54A61L27/56A61L2430/32C08L89/00C08L5/08
Inventor 张强陈纾闫书芹王秋胜韩国聪杨青青尤仁传徐安长李秀芳
Owner WUHAN TEXTILE UNIV
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