Novel reporter gene system applied to screening of biofilm inhibitor

Abstract

The invention relates to a novel reporter gene system applied to screening of a biofilm inhibitor. A bsrA gene can inhibit formation of pseudomonas aeruginosa biofilms, the encoding gene of the gene is overexpressed in bacteria and can remarkably inhibit the yield of biofilms. Therefore, with the gene as a label, the novel reporter gene system is constructed, a small molecule compound capable of activating expression of the labeled gene is screened, the labeled gene can inhibit synthesis of the biofilms from the gene level, lasting infection and antibiotic resistance caused by the biofilms canbe solved, and besides, the problems about the microbial biofilm forming mechanism in the food processing industry and inhibition and removal of the biofilms can be better solved by researching the forming mechanism of the biofilms for inhibiting the gene.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular, the invention relates to the application of a regulation gene in inhibiting microorganism biofilm (biofilm). By constructing a fusion reporter gene system to screen a small molecular compound to activate the screening marker gene, the expression of the marker gene is increased, thereby inhibiting the biofilm synthesis. It is of great significance for the multi-drug resistance caused by Pseudomonas aeruginosa and the food-borne diseases caused by biofilm. Background technique [0002] At present, the treatment of nosocomial infection mainly uses antibiotics to kill or inhibit the growth of pathogenic bacteria. However, Pseudomonas aeruginosa itself has some drug resistance mechanisms, including the outer membrane with low permeability, the expression of constitutive genes of different efflux pumps, and the production of antibiotic inactivating enzymes (such as cephalosporinase), etc.,...

AI Technical Summary

Problems solved by technology

The formation of biofilms has a serious impact on industrial environments. On the one hand, biofilms can cause a series of problems in industrial operations, such as reduced heat transfer, clogged pipes, clogged filters, and cause surface damage.

On the other hand, biofilm is very easy to form a pollution source, especially in the food industry, where bacteria adhere to food contact surfaces, providing an extremely rich source of pathogen pollution for bacterial contamination, and bringing inevitable risks to human health

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