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Preparation and application of an antifouling coating on the surface of an implantable sensor

A sensor and implantable technology, applied in the field of biomedicine, can solve the problems of decreased electrode sensing performance and use accuracy, reduced sensor life, poor biocompatibility, etc., to solve the problem of long-term sensing performance degradation, inhibition Adhesion, biocompatibility improvement effect

Active Publication Date: 2019-12-24
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to the poor biocompatibility of the subcutaneously implanted part of CGMS (i.e., the implantable glucose sensor), the sensor has a foreign body response (foreign body response, FBR) after subcutaneous implantation, including inflammation, protein adsorption, etc. , cell adhesion, blood coagulation reaction, electrodes that form fibrous capsules to wrap sensors, etc.
Biofouling around the sensor prevents the transfer of charges between the electrode and biological tissue, which reduces the sensing performance and accuracy of the electrode, and reduces the lifespan or function of the sensor.
For example, Dekang G4Platinum represents the highest level in the current CGMS industry, but its implantable sensor can only be worn for a maximum of 7 days, after which it must be replaced with a new sensor

Method used

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  • Preparation and application of an antifouling coating on the surface of an implantable sensor
  • Preparation and application of an antifouling coating on the surface of an implantable sensor
  • Preparation and application of an antifouling coating on the surface of an implantable sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of ferritin nano drug-loaded particles

[0031] In this example, using the denaturation-refolding process of ferritin, dexamethasone (abbreviated as Dex) drug molecules were entrapped in ferritin nanocages to form ferritin nano drug-loaded particles (labeled as Ft-Dex NPs) , for its preparation see figure 2 a.

[0032] Dissolve ferritin at a final concentration of 1 mg / mL in 10 mL of 8M urea and mix with stirring at room temperature 25 °C for 30 min to ensure complete unfolding of ferritin. Dexamethasone (abbreviated as Dex) was added to it with a final concentration of 0.2 mg / mL. After mixing for 15 minutes, the mixture was transferred to a dialysis bag with a molecular cutoff of 3000, and dialyzed in a refrigerator at 4°C. The dialysate is urea buffer solution (7M, 5M, 3M, 2M, 1M and deionized water) containing 0.2 mg / mL drug in gradient concentration. Each gradient concentration is dialyzed for 4-6 hours, and after dialysis, it is reassembled...

Embodiment 2

[0033] Example 2 Preparation of ferritin nano drug-loaded particles and PVA hydrogel composite coating

[0034] Adding 0.1 mL of the ferritin nano drug-loaded particle solution prepared in Example 1 into 1 mL of 3.33% PVA solution by mass percentage (the final concentration of PVA is 3% (mass percentage)), the final concentration of Dex drug in the mixed solution is 0.4 mg / mL, after thorough mixing, the mixed solution of PVA and ferritin nano drug-loaded particles was coated on the surface of the sensor by wetting and coating. Put the sensor in a -20°C refrigerator for 1 hour, thaw at room temperature for 0.5 hour, and cycle like this for 5 times, forming a composite hydrogel coating of PVA and ferritin nano drug-loaded particles on the surface of the sensor (marked as PVA+Ft- Dex NPs).

[0035] If it is necessary to prepare a two-layer composite hydrogel coating, after one freeze-thaw cycle of PVA, the solution of PVA and ferritin nano drug-loaded particles is coated again,...

Embodiment 3

[0040] The physical and chemical property evaluation of embodiment 3 coating

[0041] The thickness of different layers of PVA+Ft-DeX NPs composite coatings or drug-free PVA hydrogel coatings (marked as PVA) was characterized by a three-dimensional surface profiler (NanoFocus, Germany). The results are shown in image 3 . The thickness of the coating increases as the number of coating layers increases; there is no significant difference in the thickness of the PVA coating and the PVA+Ft-DeX NPs composite coating.

[0042] Using a scanning electron microscope (Zeiss Sigma, Germany) to observe the coating of the 6-layer PVA+Ft-DeX NPs composite coating or the coating of the PVA coating on the sensor, the results are shown in Figure 4 , indicating that different types of coatings can be coated on the surface of the sensor.

[0043] Utilize atomic force microscope (German JPK NanoWizard4) to observe the surface morphology of PVA+Ft-DeX NPs composite coating or PVA coating, the ...

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Abstract

The invention discloses preparation and an application of a stain-resistant coating on the surface of an implantable sensor and belongs to the field of biomedicines. The stain-resistant coating material is a hydrogel coating material containing ferritin drug-loaded nanoparticles and PVA and is prepared in steps as follows: ferritin is dissolved in a urea solution to be unfolded, a drug is added toa ferritin-urea mixed solution, dialysis is performed with a urea buffer solution with gradient concentration, so that ferritin is folded again, and the ferritin drug-loaded nanoparticles are obtained; the ferritin drug-loaded nanoparticles are added to a PVA solution and mixed sufficiently, and the ferritin drug-loaded nanoparticle-PVA hydrogel coating is obtained on the surface of the sensor. The coating material is used for modifying the implantable sensor, the biocompatibility of the sensor can be improved, adhesion of inflammatory cells is inhibited, fibrous capsule wrapping of the surface of the sensor is inhibited, the long-time sensing performance of the sensor in vivo is improved, and the service life of the sensor in vivo is prolonged.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the preparation and application of an antifouling coating on the surface of an implanted sensor. Background technique [0002] Diabetes is currently the third major disease that endangers human health in the world. The continuous blood glucose monitoring system (CGMS) can monitor the blood sugar changes of diabetics in real time, and can provide continuous, comprehensive and reliable blood sugar information throughout the day to understand the trend of blood sugar fluctuations. Assisting patients in real-time blood sugar regulation and adjusting treatment plans has broad application space in clinical practice. [0003] However, due to the poor biocompatibility of the subcutaneously implanted part of CGMS (i.e., the implantable glucose sensor), the sensor has a foreign body response (foreign body response, FBR) after subcutaneous implantation, including inflammation, protein adsorption,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/00A61L31/16A61L31/10
CPCA61L31/10A61L31/16A61L2300/222A61L2300/41A61L2300/606A61L2300/624C08L39/06
Inventor 雷祎凤郭赵阳张玉洁戴武斌
Owner WUHAN UNIV
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