Method for preparing medical grade valine through fermentation

A valine and pharmaceutical-grade technology, which is applied in the field of fermentation preparation and extraction of pharmaceutical-grade valine, can solve the problems of difficulty in realizing industrial scale and preparation of valine with pharmaceutical-grade purity, and achieves the extension of the acid production cycle and the promotion of Utilization and transport, the effect of less residual sugar

Active Publication Date: 2018-07-17
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some of the above methods are difficult to prepare valine with pharmaceutical grade purity, and some are difficult to realize industrial scale

Method used

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  • Method for preparing medical grade valine through fermentation
  • Method for preparing medical grade valine through fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for preparing and extracting pharmaceutical grade valine by fermentation, comprising the steps of:

[0039] Brevibacterium lactofermentum ATCC1470 seed liquid (1 × 10 8 cfu / ml) was inserted into the fermentation medium according to the inoculation amount of 7%, and fermented continuously for 55 hours to obtain a valine fermentation liquid; the temperature during the fermentation process was controlled at 30°C, the pH was controlled at 6.5, and the glucose concentration was controlled not less than 20g / L;

[0040] The components of the fermentation medium are: glucose 50g / L, corn steep liquor 30g / L, soybean meal 10g / L, lactose 10g / L, ammonium sulfate 6g / L, potassium dihydrogen phosphate 0.1g / L, magnesium sulfate 0.1g / L, manganese sulfate 6mg / L, ferrous sulfate 6mg / L, potassium chloride 6mg / L;

[0041] Utilize the ceramic membrane to filter the valine fermented liquid to obtain filtrate A (valine content is 4.9%) and wet thalline; Add 3wt% tourmaline powder to...

Embodiment 2

[0049] A method for preparing and extracting pharmaceutical grade valine by fermentation, comprising the steps of:

[0050] Brevibacterium lactofermentum ATCC1470 seed liquid (1 × 10 8 cfu / ml) was inserted into the fermentation medium according to the inoculation amount of 8%, and fermented continuously for 50 hours to obtain valine fermentation broth; the temperature during the fermentation process was controlled at 30°C, the pH was controlled at 6.5, and the glucose concentration was controlled not less than 15g / L;

[0051] The components of the fermentation medium are: glucose 50g / L, corn steep liquor 30g / L, soybean meal 10g / L, lactose 10g / L, ammonium sulfate 6g / L, potassium dihydrogen phosphate 0.1g / L, magnesium sulfate 0.1g / L, manganese sulfate 6mg / L, ferrous sulfate 6mg / L, potassium chloride 6mg / L;

[0052] Utilize ceramic membrane to filter the valine fermented liquid to obtain filtrate A (valine content is 4.7%) and wet thalline; Add 3wt% tourmaline powder to wet th...

Embodiment 3

[0060] 1. Taking Example 1 as an example, the content of valine was detected, and the content of valine in the filtrate B was detected respectively; a control group was also set at the same time, wherein, the control group 1 was: only dialysis culture treatment was carried out, and no electric Stone powder and heat treatment, the rest are the same as in Example 1; Control group 2: After the fermentation is completed, tourmaline powder and dialysis culture treatment are carried out, without heat treatment, the rest are the same as in Example 1; Control group 3: After the fermentation is completed, heat treatment and dialysis culture treatment are carried out , do not adopt tourmaline powder to process, and all the other are with embodiment 1; Detect the content of valine in each group filtrate B, and concrete results are shown in Table 1:

[0061] Table 1

[0062]

[0063] Conclusion: As shown in Table 1, through the recultivation treatment of discarded bacterial strains, a ...

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Abstract

The invention belongs to the technical field of biochemical engineering and particularly relates to a method for preparing medical grade valine through fermentation. The method comprises the followingsteps: fermenting, filtering and concentrating to prepare coarse valine; performing primary acid dissolution on the coarse valine by adopting hydrochloric acid, cooling, crystallizing and centrifuging to obtain the valine solid and mother solution; performing secondary acid dissolution on the valine solid with hydrochloric acid, cooling, crystallizing and centrifuging to obtain the valine solid and mother solution again; dissolving the obtained valine solids with water by adopting distilled water, performing ion exchange, discoloration, refined filtration and crystallization, so as to obtainthe medical grade valine. According to the method disclosed by the invention, the fermentation efficiency is high, heteroacid can be effectively removed, the product quality is greatly improved, and the medical grade standard is reached.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and specifically provides a method for preparing and extracting pharmaceutical grade valine by fermentation. Background technique [0002] The chemical name of valine is 2-amino-3-methylbutyric acid, and the molecular formula is C 5 h 11 NO 2 , the relative molecular mass is 117.15. Valine is one of the eight essential amino acids in the human body. It has a variety of physiological functions and plays an important role in many aspects such as metabolic regulation and information transmission in human life. In the field of medicine, valine is mainly used to configure amino acid infusion, which can treat blood-brain barrier, chronic liver cirrhosis, hepatic coma, renal failure, congenital metabolic defects and diabetes. Valine works with isoleucine and leucine to promote normal body growth, repair tissue, and regulate blood sugar. [0003] The production methods of valine include...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C07C227/40C07C229/08
CPCC07C227/40C12P13/08C07C229/08
Inventor 李学朋杨瑞丽张宗华王绍冰庄会华刘元涛
Owner XINJIANG FUFENG BIOTECH
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