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Compound bacterial agent for aquaculture and application thereof

A compound bacterial agent, aquaculture technology, applied in the application, bacteria, fungi and other directions, can solve the problems of difficult to guarantee product quality, limited types and quantities of metabolites, and reduced activity.

Active Publication Date: 2018-07-13
北京市科学技术研究院资源环境研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microbial preparations are greatly affected by temperature and external conditions. Most of the existing lactic acid bacteria series are fermented at a single temperature, the types and quantities of metabolites are limited, the shelf life is short, the activity continues to decrease during storage, and the product quality is difficult to guarantee.

Method used

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  • Compound bacterial agent for aquaculture and application thereof
  • Compound bacterial agent for aquaculture and application thereof
  • Compound bacterial agent for aquaculture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the production method of aquaculture compound bacterial agent

[0027] 1. Aquaculture compound bacterial agent method: (1) inoculate the four kinds of bacteria described in claim 2 on solid medium for activation; (2) pick single colonies of the four activated strains and inoculate them respectively Cultivate for 48 hours on the respective liquid culture medium at 37°C; (3) Inoculate the cultured strains in (2) into the fermenter according to the ratio in claim 2 and ferment for 24 hours at 37°C , made into a bacterial suspension, the number of viable bacteria in the bacterial solution was 6×10 9 CFU / mL, pH3.8, that is, the preparation of the complex bacterial agent fermentation is completed.

[0028] 2. The production method of the compound expansion culture agent product (take the expansion culture 100 L as an example): (1) Move the 100 liter plastic white bucket to an outdoor place exposed to direct sunlight, and add about 50 L of clean water into the ...

Embodiment 2

[0029] Embodiment 2: the impact of aquaculture compound bacterial agent on the water quality of artificially built cement culture ponds

[0030] 1. The basic situation before the application of the inoculum agent in the pond of an aquaculture base:

[0031] The selected No. 5 pond and No. 7 pond have a water depth of 1.5-2m and a water surface of about 2 mu / pond. The bottom of the pond is cement. When the test started on August 1, the average size of the fish in the two ponds was between 350g and 400g, the amount of feed fed was 20kg / day / pond, and the protein content of the feed was about 45%. The measured original nitrite values ​​were respectively The pH values ​​were 1.16mg / L and 1.06mg / L, respectively, and the pH values ​​were 8.79 and 8.56. The blue-green algae outbreak was serious. Pond No. 10 was selected as a blank, and the pond without fish fry was not sprayed with bacterial agents and feed. The original pH test value on August 1 was 8.80, and nitrite was less than 0...

Embodiment 3

[0037] Embodiment 3: the impact of aquaculture compound bacterial agent on the water quality of traditional soil pond culture pond

[0038] 1. The basic situation before the application of the inoculum agent in the pond of an aquaculture base:

[0039] Three ponds, North No. 8 Pond, North No. 10 Pond, and South No. 2 Pond, were selected as the test ponds. The conditions of the ponds are roughly as follows: North No. 8 Pond, with a water area of ​​10 mu and a water depth of 2 meters. The average size of fish is 200-250g About 15,000 fish in number; North No. 10 pond, with a water area of ​​12 acres, a water depth of 2 meters, a fish size of about 300 grams, and a quantity of 16,000 fish; South 2 Haotang, a water area of ​​6 acres, a water depth of 2 meters, a quantity of 13,000 fish, and a size of 300 grams about. The species of the fish are all white fish, the feeding amount is 2% of the total weight of the fish per day, and the protein content in the feed is about 40%. The ...

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Abstract

The invention discloses a fermentation method, an expansion method and the application of a novel compound bacterial agent for aquaculture and belongs to the technical field of feed additives, aquaculture water quality improvement and microorganisms. The compound bacterial agent for aquaculture is prepared by lactobacillus plantarum, leuconostoc mesenteroides, pediococcus acidilactici and pichia pastoris. The compound bacterial agent disclosed by the invention can promote the intestinal flora balance and the disease resistance of fishes, shrimps and crabs through mixing fish, shrimp and crab feed and meanwhile can enhance the appetite of the fishes, the shrimps and the crabs and improve the feed utilization rate; through spraying on aquaculture water according to a certain proportion, theeffects on prevention and inhibition of cyanobacteria, degradation of pH and nitrite and promotion of the ecological balance of the aquaculture water are good. The compound bacterial agent disclosed by the invention is used in the aquaculture and is short in fermentation period, wide in suitable temperature range, stable in activity, simple in process, less in equipment investment, easy to implement and suitable for farmers to produce and use.

Description

technical field [0001] The invention discloses a method for fermenting and expanding cultivation of a novel composite bacterial agent for aquaculture and its application, and belongs to the technical fields of feed additives, water quality improvement of aquaculture water and microbes. Background technique [0002] China is currently the largest aquatic product farming country in the world, and it is also the only country in the world where aquaculture production exceeds fishing production. In order to avoid polluting the water environment and ensure the healthy and sustainable development of the aquaculture industry, my country's aquaculture industry will transform from a quantitative model to a quality-benefit model. The large-scale and intensive farming model will gradually replace the original extensive farming model. High-density farming During the process, bait, space competition, and the accumulation of residual bait and feces directly lead to the deterioration of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34A23K10/18C12R1/25C12R1/01C12R1/84
CPCA23K10/18C02F3/34C02F3/347C12N1/16C12N1/20
Inventor 金京华杨艳程言君其他发明人请求不公开姓名
Owner 北京市科学技术研究院资源环境研究所
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