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Trichoderma asperellum and application thereof in lead-polluted soil repairing

A technology for Trichoderma aculeatus and polluted soil, which is applied in the field of agricultural microorganisms and ecological restoration, can solve the problems of consuming large energy or chemical products, restoring the adverse effects of soil physical and chemical properties of polluted soil, and high finished products, and achieves excellent tolerance and adsorption capacity. , broad heavy metal tolerance, reducing the effect of stress

Active Publication Date: 2018-07-13
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the remediation technologies for lead-contaminated soil mainly include physical and chemical remediation, phytoremediation, and microbial remediation. Among them, the traditional physical and chemical remediation methods have faster results, but they require a large amount of engineering, consume a lot of energy or chemicals, etc., and the finished product is relatively high. Moreover, the use of some passivating agents may even cause the restoration of contaminated soil and have adverse effects on the physical and chemical properties of the soil, thereby affecting the subsequent use of the soil; the operation of phytoremediation is inconvenient; and the use of microbial remediation has broad application prospects

Method used

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  • Trichoderma asperellum and application thereof in lead-polluted soil repairing
  • Trichoderma asperellum and application thereof in lead-polluted soil repairing
  • Trichoderma asperellum and application thereof in lead-polluted soil repairing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation and identification of bacterial strains

[0028] 1. Isolation of strains:

[0029] Select 10g of soil collected from a polluted area of ​​a lead storage battery factory in Shandong and put it in a triangular flask. Under sterile conditions, add 90ml of sterile water, shake fully to fully elute the microorganisms from the soil, then let it stand for 20 minutes, and absorb the supernatant Dilute 100 times with sterile water; take 1ml of the supernatant and evenly spread it on a solid plate (Martin's medium) with a lead concentration of 100mg / L. 100-1500mg / L Martin's medium, 30 ℃ gradient acclimatization culture; the colony with the strongest resistance to lead was selected, and the single colony was continuously purified by plate streak separation method, and the best resistance was screened. Lead-competent strains were used as test strains for further study.

[0030] 2. Identification of strains:

[0031] (1) Morphological identification:

[0032...

Embodiment 2

[0045] Embodiment 2: Determination of tolerance of Trichoderma asperellum to other heavy metals

[0046] Trichoderma asperellum (Trichoderma asperellum) screened and isolated in Example 1 was inoculated on the glucose-peptone medium containing heavy metals (Pb, Cd, Cu and Zn), and the concentration of heavy metals was gradually increased until the bacterial strain could not grow normally. This concentration was The minimum inhibitory concentration, the results are shown in Table 1.

[0047] Table 1: Minimal inhibitory concentrations of Pb, Cd, Cu and Zn against strains

[0048]

[0049] As can be seen from Table 1, Trichoderma asperellum of the present invention has a very good tolerance to the heavy metal Pd, and at the same time, it also has a certain tolerance to heavy metals such as Cd, Cu, and Zn.

Embodiment 3

[0050] Embodiment 3: Determination of removal and adsorption capacity of Trichoderma asperellum to lead ion

[0051] In 100ml lead solution, the lead concentrations are 50, 100, 150, 200, 250, 300, 350, 400, 450, 500mgL -1 Add the Trichoderma asperellum (Trichoderma asperellum) that 0.2g embodiment 1 screens and separates respectively in the Erlenmeyer flask of 0.2g, at 150rpm min -1 , Shake at a constant temperature at 30°C for 24 hours, filter the solution after the shaking is completed, and measure the lead content in the solution.

[0052] The removal rate and adsorption capacity of Trichoderma aculeatus to lead ions were calculated according to the following formula.

[0053] Removal rate (η%) = (C 0 -C E )*100% / C 0 ;

[0054] Adsorption capacity (Q) = (C 0 -C E )*V / 1000W;

[0055] In the formula: the adsorption capacity of Q-microbes to lead (mg / g); C 0 -Initial concentration of lead ions (mg / L); C E - lead concentration (mg / L) when the adsorption reaches equil...

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Abstract

The invention discloses trichoderma asperellum. The strain is collected in the CGMCC on January 22, 2018, with the biological collection number of CGMCC NO.15266. The trichoderma asperellum obtained by screening and separation is excellent in tolerance and adsorption capacity for various heavy metals, particularly for lead; the trichoderma asperellum is easy to colonize and survival in heavy metal-polluted soil, particularly in lead-polluted soil, and is suitable for microbial repairing for the soil.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms and ecological restoration, in particular to a strain of Trichoderma aculeatus and its application in the restoration of lead-contaminated soil. Background technique [0002] Lead is a widely distributed and highly accumulative environmental pollutant in heavy metal polluted soil. There are varying degrees of lead pollution in various regions of our country, and lead poisoning incidents also occur frequently. Lead pollution exists in air, water and soil and other environmental elements, but compared with air and water, lead pollution in soil is more common. Most of the lead that enters the soil exists in the topsoil layer and hardly moves downward. Lead is easy to combine with organic matter in the soil, and it is extremely difficult to dissolve. Excessive lead will hinder the growth and development of crops, mainly manifested as the decrease of chlorophyll, hindering the re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10A01N63/04A01P21/00C12R1/885
CPCA01N63/30B09C1/10C12N1/14C12N1/145C12R2001/885
Inventor 诸葛玉平孙鑫娄燕宏崔秀敏宋付朋王会韩飞王婷婷
Owner SHANDONG AGRICULTURAL UNIVERSITY
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