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Spiral-type variable-cross-section micro fluidic PCR chip, and manufacturing method thereof

A variable cross-section, helical technology, applied in chemical instruments and methods, biochemical equipment and methods, enzymology/microbiology devices, etc. The effect of reducing demand and realizing real-time detection

Active Publication Date: 2018-07-13
NAT UNIV OF DEFENSE TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In addition, Shanghai Yimu Company has also developed a MF-100 microfluidic PCR instrument, but this product has not been listed, and there is no relevant technical parameter report

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  • Spiral-type variable-cross-section micro fluidic PCR chip, and manufacturing method thereof
  • Spiral-type variable-cross-section micro fluidic PCR chip, and manufacturing method thereof
  • Spiral-type variable-cross-section micro fluidic PCR chip, and manufacturing method thereof

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Embodiment Construction

[0034] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. The components of the embodiments of the invention generally described and illustrated in the figures herein may be arranged and designed in a variety of different configurations. Accordingly, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts shall fall within the protection scope of the present invention.

[0035] In the description of the pres...

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Abstract

The invention provides a spiral-type variable-cross-section micro fluidic PCR chip, and a manufacturing method thereof. The spiral-type variable-cross-section micro fluidic PCR chip comprises a reaction system and a temperature control system; the reaction system comprises a base sheet and a cover sheet connected via bonding; a base sheet channel is arranged in the base sheet; the base sheet channel comprises a constant cross section channel and a spiral-type variable-cross-section channel; the spiral-type variable-cross-section channel is designed to be spiral taking the center of the base sheet as the circle center; the size of the cross section of the spiral-type variable-cross-section channel is reduced gradually with increasing of the length. The spiral-type variable-cross-section micro fluidic PCR chip is small in size, is portable, is capable of realizing high degree integration of a plurality of PCR reaction auxiliary equipment. According to the spiral-type variable-cross-section micro fluidic PCR chip, the base sheet can be divided into three temperature zones; three temperature zone constant temperature independent control is adopted by the temperature control system, andthe uniformity control of the temperature zones is improved using thermal conductive elements, so that requirements of the PCR reaction on high speed accurate temperature increasing and reducing control of the temperature control system can be reduced.

Description

technical field [0001] The invention relates to the technical field of polymerase chain (PCR) amplification reaction, in particular to a spiral variable cross-section microfluidic PCR chip and a manufacturing method thereof. Background technique [0002] Polymerase chain reaction (PCR) is a technique for selectively amplifying DNA or RNA sequences in vitro. The basic reaction principle is a process of repeated DNA template unzipping, primer and template DNA binding, and DNA polymerase catalyzing the formation of new DNA strands. Repeating this process continuously can make the target DNA fragment exponentially amplify. The essence of the PCR amplification reaction is the reaction of the reaction solution in three different temperature zones, including denaturation (dsDNA denatures into ssDNA at 92-96°C), annealing (primers bind to the complementary region of the template at 45-72°C), extension (in the At 72°C, TaqDNA polymerase catalyzes the DNA template-primer conjugate an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/00
CPCB01L3/502792B01L7/52
Inventor 董培涛吴学忠何昱王朝光张晨煜陈剑
Owner NAT UNIV OF DEFENSE TECH
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