Chromosome configuration acquisition library and construction method thereof

Abstract

The invention discloses a chromosome configuration acquisition library and a construction method thereof. The construction method comprises preparing a single cell suspension from a tissue sample, carrying out cross-linking fixation on the single cell suspension to obtain a DNA-protein cross-linking complex, acquiring a DNA fragment from the DNA-protein cross-linking complex and constructing the chromosome configuration acquisition library. The tissue sample is processed into the single cell suspension and the single cell suspension is fixed through cross-linking so that the tissue cross-linking fixation effects are seriously affected by the tissue size, the excessive fixation of partial tissue cross-linking is avoided, a small fragment ratio is increased in the library and the interactioninformation between DNA and protein in the chromosome configuration is reduced. The method solves the problem that the cell morphology change causes low chromosome configuration acquisition accuracy,the stability and universality of the multi-tissue library are improved and the labor and reagent costs are greatly saved.

Description

technical field [0001] The invention relates to the field of high-throughput sequencing library construction, in particular to a chromosome configuration capture library and a construction method thereof. Background technique [0002] With the development of sequencing technology, human beings have more and more thorough understanding of biological species information, but resequencing is usually based on the reference genome for variation information detection, and chromosomes are carriers of genetic material, and their activity and function are determined by structure and spatial conformation Co-determined, the three-dimensional conformation of chromosomes has also become a representative technology for genome research. Hi-C technology is derived from the Chromosome conformation capture (3C) technology, which takes the entire nucleus as the research object, uses high-throughput sequencing technology, and combines biological The information analysis method studies the relat...

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Problems solved by technology

[0042] The main purpose of this application is to provide a chromosome configuration capture library and its construction method to improve the existing tissue-based chromosome configuration capture method is difficult to effectively build the defect

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