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Inoculant, feed or additive and removal method for vomitoxin

A technology of vomitoxin and additives, applied in the field of microorganisms, to achieve the effects of removing safety, ensuring safe production, and improving utilization

Active Publication Date: 2018-07-06
COFCO NUTRITION & HEALTH RES INST +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous emergence of the disadvantages of physical and chemical detoxification methods and the increasing awareness of the advantages of rapidly developing biotechnology, research on the use of microorganisms for toxin detoxification is gradually being carried out at home and abroad, although there are not many research results on the use of microorganisms for detoxification , but the existing achievements have shown good development and application prospects

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  • Inoculant, feed or additive and removal method for vomitoxin
  • Inoculant, feed or additive and removal method for vomitoxin

Examples

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preparation example Construction

[0028] The present invention also provides a preparation method of the above-mentioned inocula, the method comprising: respectively inoculating the above-mentioned yeast and bacillus into the corresponding medium for culturing, and then making the obtained culture into dry powder according to the above ratio After compounding, the bacterial agent of the present invention is obtained.

[0029] In the present invention, the method of inoculating the yeast and bacillus into the culture medium for culturing can be changed in a wide range. For example, the method may include: cultivating the yeast and the bacillus in separate culture systems; and The yeasts and bacilli obtained by culturing separately are made into dry powder and then mixed according to the ratio to obtain the microbial inoculum. The method of making dry powder is well known to those skilled in the art, and will not be repeated here.

[0030] In the second aspect, the present invention provides the application of the a...

Embodiment

[0050] Hereinafter, the present invention will be described in detail through examples, but the present invention is not limited thereby. In the following preparation examples, examples and comparative examples, unless otherwise specified, all materials are commercially available, and all methods are conventional methods in the art.

[0051] YPD medium: 10g yeast extract, 20g peptone, add water to 900ml, sterilize at 115℃ for 20min, then add 100ml of sterilized glucose solution (concentration 20g / 100mL) and mix well.

[0052] LB medium: 5 grams of beef extract, 10 grams of peptone, 5 grams of sodium chloride, add water to 1000 ml, sterilize at 115°C for 20 minutes, pH 7.0-7.2.

[0053] Trichosporon glutinosa CICC 32858, Yarrowia lipolytica CICC 1441, Candida utilis CICC 1314, Brevibacterium casei CICC 23944 were all purchased from the China Industrial Microbial Culture Collection and Management Center.

[0054] Bacillus subtilis BNCC188062 and Bacillus licheniformis BNCC337190 were pu...

preparation example

[0056] The activated yeast was inoculated into liquid YPD medium at an inoculum of 1% by volume, and cultured at 150 rpm and 30°C. The culture time was such that the yeast concentration was (1±0.1)×10 9 CFU / ml. The activated bacilli were inoculated into liquid LB medium with an inoculum of 1% by volume, and cultured at 150 rpm and 30°C. The culture time was such that the concentration of bacilli was (1±0.1)×10 9 CFU / ml. Then they are made into dry powders, and the total number of viable bacteria contained in each gram of each dry powder is (1±0.1)×10 9 CFU, and prepare the compound bacterial agent according to the various ratios described in Table 1.

[0057] Table 1

[0058]

[0059]

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Abstract

Relating to the field of microorganisms, the invention discloses an inoculant, a feed or additive and a removal method for vomitoxin. The inoculant includes yeast and bacillus in a dry weight ratio of1:(0.5-15). According to the invention, the combined use of yeast and bacillus can efficiently remove vomitoxin, and the inoculant provided by the invention can be easily processed or added into vomitoxin contaminated cereals and / or feed, and then can effectively lower the content of vomitoxin in vomitoxin contaminated cereals and / or feed, can increase the utilization rate of cereals and / or feed,is beneficial to the health of raised animals, and can ensure the safety production of animal husbandry.

Description

Technical field [0001] The present invention relates to the field of microorganisms, in particular to a bacterial agent and its application in removing vomiting toxins, a feed or additive and a method for removing vomiting toxins. Background technique [0002] Vomitoxin, also known as deoxynivalenol (DON), has the chemical name 3α,7α,15-trihydroxyfusarium-9-en-8-one, because it can cause pigs The vomiting is named after Fusarium graminearum and Fusarium culmorum which infect wheat, barley, oats, corn and other grains to produce trichothecenes. All over the world, vomiting toxin is one of the main mycotoxins that contaminate grain, feed, and food, which seriously affects the health of people and livestock. After humans and animals ingest food contaminated with vomiting toxins, they can cause anorexia, vomiting, diarrhea, fever, unstable standing, unresponsiveness and other acute symptoms of poisoning. In severe cases, they damage the hematopoietic system and cause death. The seri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/16A23L5/20A23K10/18
CPCA23V2002/00C12N1/16C12N1/18C12N1/20A23V2250/76
Inventor 林海龙苏会波李文钊谭剑熊强李凡陈博佟易李义李久仁樊维荣朱镜羲刘劲松杨勇范洪岩靳玉双
Owner COFCO NUTRITION & HEALTH RES INST
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