A preparation method of molecularly imprinted polymer microspheres for the detection of 2,4,6-trinitrophenol

Abstract

A method for preparing molecularly imprinted polymer microspheres for 2,4,6-trinitrophenol detection, characterized in that: 2,4,6-trinitrophenol and N,N'-diisopropyl The imprinted molecules of polynitrophenol derivatives produced by the carbodiimide reaction have bright yellow fluorescence, and their fluorescence has opening and closing characteristics under acid-base conditions, and the elution in the molecularly imprinted polymer microspheres is located in the imprinted layer The imprinted molecules of the imprinted layer have a cavity structure complementary to the structure, size and functional groups of the imprinted molecules, and the polymer microspheres that elute the imprinted molecules have specific recognition sites for target analyte molecules, and the target molecules enter The specific recognition site realizes the selective recognition of the target analyte molecule, and realizes the sensitive detection through the opening and closing of the fluorescence of the target molecule. The specific surface area of ​​the molecularly imprinted microsphere is large, and the spatial structure of the recognition site is stable and effective. Multiple spots, highly selective recognition and highly sensitive detection of target molecules.

Description

technical field [0001] The invention relates to the field of material science, in particular to a preparation method for detecting 2,4,6-trinitrophenol molecular imprint. Background technique [0002] International relations in today's world are complex and changeable, and terrorist and violent incidents such as bombings occur frequently, which seriously endanger the lives and property safety of people all over the world and social stability, and pose a threat to the security and stability of countries and regions. Therefore, the detection and analysis of explosives has attracted great attention from research institutions in various countries, trying to predict the existence of explosives and reduce the harm of explosives to humans through analysis and detection technology. 2,4,6-trinitrophenol (2,4,6-trinitrophenol, TNP) belongs to phenolic substance, also known as picric acid (picric acid, PA). Its raw materials are easy to obtain, its preparation is simple, and its explo...

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Problems solved by technology

[0010] Although the above methods have their own characteristics, there is no report in the literature that the target molecule itself has fluorescent properties, and the target molecule with yellow fluorescence enters the recognition site through its own optical signal sensitive output.

At the same time, there is no report in the literature that the polynitrophenol derivatives with bright yellow fluorescence produced by the reaction of 2,4,6-trinitrophenol and N,N'-diisopropylcarbodiimide are imprinted into polymer microparticles. Spheres, imprinted derivatives that enter the recognition site, enable reporting of recognition and detection by switching on and off of autofluorescence

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