Live vaccine diluent as well as preparation method and application thereof and vaccine product

A live vaccine and diluent technology, applied in the field of vaccines, can solve the problems of being susceptible to maternal antibody interference, short antibody maintenance time, and long production time, so as to improve animal antibody content, reduce infection and morbidity, and be easy to operate. Effect

Active Publication Date: 2018-07-06
SICHUAN ANIMAL SCI ACAD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is in order to overcome above-mentioned prior art defect, provides a kind of live vaccine diluent, easily suffers maternal antibody interference when immunizing animal live vaccines such as swine fever, porcine pseudorabies, the antibody titer after immunization is low, produces Long time, short antibody maintenance time and other issues

Method used

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  • Live vaccine diluent as well as preparation method and application thereof and vaccine product
  • Live vaccine diluent as well as preparation method and application thereof and vaccine product
  • Live vaccine diluent as well as preparation method and application thereof and vaccine product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present embodiment provides a kind of live vaccine diluent, comprising:

[0034] Carbomer, levamisole and ginsenoside are dissolved in normal saline, and the control final concentration is: carbomer 1% (W / V), levamisole 5% (W / V) and ginsenoside 0.01% (W / V ), using sodium hydroxide or hydrochloric acid to adjust the pH to 7.2 to obtain a live vaccine dilution.

[0035] Dispense according to a certain volume and sterilize under high pressure at 121°C for 15 minutes before use.

Embodiment 2

[0037] The present embodiment provides a kind of live vaccine diluent, comprising:

[0038] Carbomer, levamisole and notoginsenoside are dissolved in water, and the control final concentration is: carbomer 0.02% (W / V), levamisole 1% (W / V) and notoginsenoside 0.001% (W / V ), using sodium hydroxide or hydrochloric acid to adjust the pH to 7.0 to obtain a live vaccine dilution.

[0039] Dispense according to a certain volume and sterilize under high pressure at 121°C for 20 minutes before use.

Embodiment 3

[0041] This embodiment provides a kind of live vaccine diluent, and it contains: 0.01% (W / V) carbomer, 0.1% (W / V) levamisole, 0.0001% (W / V) soybean saponin, 100mM sodium chloride , 0.5mM potassium chloride, 100mM disodium hydrogen phosphate and 0.1mM potassium dihydrogen phosphate.

[0042] The preparation method of this live vaccine diluent is:

[0043] According to the formula, the above substances are dissolved in water for injection, and sodium hydroxide is added to adjust the pH to 7.2. After being dispensed according to a certain volume, it is autoclaved at 121°C for 25 minutes and then used for later use.

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Abstract

The invention discloses a live vaccine diluent as well as a preparation method and application thereof and a vaccine product, which relates to the field of vaccines. The live vaccine diluent disclosedby the invention is prepared from the following components: 0.01 to 1 percent of (W / V) Carbomer, 0.1 to 5percent of (W / V) levamisole as well as 0.0001 to 0.01 percent of (W / V) saponin. The live vaccine diluent can solve the problems that an animal live vaccine for immunizing swine fever, porcine pseudorabies and the like is susceptible to the infection of a maternal antibody, the titer of the antibody after the immunization is low, the production time is long, the maintenance time of the antibody is short and the like.

Description

technical field [0001] The invention relates to the field of vaccines, in particular to a vaccine diluent, its preparation method, application and vaccine products. Background technique [0002] Vaccination is the best way to prevent and control infectious diseases. The vaccine strains currently used in the market are mainly classified into two categories, one is attenuated live vaccines, and the other is inactivated vaccines or subunit vaccines. Among them, the attenuated live vaccine enters the animal body after immunization and can infect host cells to continue to replicate. In clinical practice, piglets can obtain passive immunity through colostrum after birth. In the case of passive immunity, the maternal antibodies obtained by piglets will interfere with the replication of the attenuated live vaccine in the body, resulting in immune failure. In a group, it is often difficult to find a balance point due to individual differences, so multiple immunizations are required...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K47/32A61K31/429A61K31/704A61P31/14A61P31/22
CPCA61K31/429A61K31/704A61K39/39A61K47/32A61K2039/552A61K2039/55511A61K2039/55577A61K2300/00
Inventor 周远成蔡雨函牛婷代洪波李碧钟颖卓秀萍廖果林燕王雪涛
Owner SICHUAN ANIMAL SCI ACAD
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