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Vaccine composition, and preparation method and application thereof

A technology of vaccine composition and Muscovy duck parvovirus, which is applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., can solve the problems such as the impact of the immune effect of the attenuated live vaccine

Inactive Publication Date: 2015-01-21
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the ubiquity of maternal antibodies against Muscovy duck parvovirus in ducklings, the immune effect of the attenuated live vaccine will be greatly affected

Method used

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  • Vaccine composition, and preparation method and application thereof
  • Vaccine composition, and preparation method and application thereof
  • Vaccine composition, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation and Application of Muscovy Duck Parvovirus M91-G27 Strain Antigen-Antibody Complex Vaccine

[0037] 1.1 Preparation of Muscovy duck parvovirus M91-G27 strain antigen

[0038] 1.1.1 Preparation of whole virus antigen of Muscovy duck parvovirus M91-G27 strain

[0039]Take the Muscovy duck parvovirus M91 strain and use the published method (Wang Yongkun, Qian Zhong, Qian Shumei, etc. Comparison of the characteristics of duckling duck parvovirus and gosling plague virus and the application of dual live vaccines[J], China Poultry Industry Guide, 2004, 21 (9): 18-20), first passed 3 generations in muscovy duck embryos, named M91 strain, and then continuously passed 27 generations in 12-day-old goose embryos, named M91-G27 strain. The M91-G27 strain was inoculated with goose embryos for propagation, and the infected goose embryo liquid was harvested to prepare the whole virus antigen of Muscovy duck parvovirus M91-G27 strain. The viral content of the wh...

Embodiment 2

[0099] Example 2 Preparation and Application of Muscovy Duck Parvovirus P1 Strain Antigen-Antibody Complex Vaccine

[0100] 2.1 Preparation of Muscovy duck parvovirus P1 strain antigen

[0101] 2.1.1 Preparation of whole virus antigen of Muscovy duck parvovirus P1 strain

[0102] Take Muscovy duck parvovirus P1 strain seed virus, using the published method (Fujian Academy of Agricultural Sciences Institute of Animal Husbandry and Veterinary Medicine, Preparation method of new Muscovy duck parvovirus vaccine: China, 101880651A[P], 2010.11.10), seed the virus according to Muscovy duck embryo fibroblasts were inoculated synchronously with 1% of the culture solution, rotated at 37°C until the cytopathic rate reached more than 75%, and the cytotoxicity was harvested to make the whole virus antigen of Muscovy duck parvovirus P1 strain. The viral content of the whole virus antigen was determined to be 10 6.0 ELD 50 / 0.2ml.

[0103] 2.1.2 Eukaryotic expression of VP2 protein of Mu...

Embodiment 3

[0163] The preparation of embodiment 3 muscovy duck parvovirus Q strain antigen-antibody complex vaccine

[0164] 3.1 Preparation of Muscovy Duck Parvovirus Q Strain Antigen

[0165] 3.1.1 Preparation of whole virus antigen of Muscovy duck parvovirus Q strain

[0166] Take Muscovy duck parvovirus Q strain seed virus, using the published method (Lou Hua, Wang Zhengfu, Xu Bin. Development and application of Muscovy duck parvovirus attenuated vaccine [J], China Veterinary Science and Technology, 1998, 28(3): 24-25), the virus was properly diluted with sterilized saline, inoculated with muscovy duck embryos for continuous passage, when the virus content reached 10 5 ELD 50 When it is more than 0.2ml, it can be used as the whole virus antigen of the Q strain of parvovirus. The viral content of the whole virus antigen was determined to be 10 6.4 ELD 50 / 0.2ml.

[0167] 3.1.2 Eukaryotic expression of Muscovy duck parvovirus Q strain VP2 protein

[0168] Infected duck embryo fl...

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Abstract

The invention provides a vaccine composition, comprising an immune amount of muscovy duck Parvovirus antigen and an immune amount of muscovy duck parvovirus antibody. The vaccine composition can be applied to immunization of young muscovy duck, also can be directly applied to immunization of muscovy duck embryo; the immunization effect is not affected by maternal antibody, and quickly produces immunity, and ideal protection can be produced of the in the second day after immunization; attacked by strong virus, the immune ducks 5 / 5 have no morbidity, and control ducks 5 / 5 are dead.

Description

technical field [0001] The invention relates to a vaccine composition for preventing parvovirus disease of Muscovy ducks. Background technique [0002] Muscovy duck parvovirus disease is an infectious disease of young Muscovy ducks caused by Muscovy duck parvovirus (MPV). The disease mainly affects young Muscovy ducks aged 1 to 3 weeks, commonly known as "three-week disease". The morbidity rate is 26% to 62%, and the fatality rate is 22% to 43%. Most of the recovered ducks become stiff ducks, which brings certain losses to the duck industry. The disease is highly contagious, and the main symptoms are diarrhea, panting, progressive weight loss and weak feet. [0003] At present, the prevention of Muscovy duck parvovirus disease mainly uses the attenuated live vaccine of Muscovy duck parvovirus disease. The attenuated live vaccine has been widely used because of its fast immunity. However, because the live vaccine is greatly affected by the maternal antibody, and the young m...

Claims

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Application Information

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IPC IPC(8): A61K39/42A61P37/04A61P31/20A61K39/23
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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