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Tissue culture, propagation expanding and seedling growing method of dendrobium strongylanthum

A technology of tissue culture and tissue culture of Dendrobium comb-lipped, which is applied in the field of tissue culture expansion and breeding of Dendrobium comb-lipped, can solve the problems of the reduction of wild resources of Dendrobium comb-lipped, difficult germination and growth, and few breeding seedlings, so as to ensure the quality and variety of seedlings. Pure, power-saving effect

Inactive Publication Date: 2018-07-06
康美药业(昆明)种质资源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the Dendrobium chinensis circulating in the domestic market is mainly collected from wild resources in the Pu'er area of ​​Yunnan Province. There is no artificial cultivation. Generally, it is propagated by division and cuttings. There are few seedlings, low reproduction rate, and long growth cycle. , the probability of fruiting without artificial pollination in a normal growth environment is small; due to the constraints of many factors such as few fruit pods and small fruit powder, it is difficult to germinate and grow in a natural environment, and the endless excavation of wild resources has led to the wild resources of Dendrobium spp. gradually decreases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for tissue culture expansion and breeding of dendrobium comb lipped dendrobium comprises the following steps:

[0037] The steps of explant selection: select the female parent plant of robust Dendrobium spp. for 3 years; artificially pollinate the Dendrobium spp. during the flowering period in late September every year, let it grow fruit pods, and after 3-4 months carefully Cultivate. When the pods are mature, pick the mature pods and store them in the refrigerator at 3-7°C for 2 weeks; when the pods are mature, the outer seed shell is yellow-green and intact.

[0038] Steps for disinfection of explants: Take out the mature fruit pods, soak them in detergent water or washing powder water for 18 minutes, brush the surface of the seed shells with a brush, and rinse them with tap water for 10 minutes; room, in a sterile environment, soak it in medical alcohol with a volume concentration of 75% for 50s, stir it with tweezers during the soaking period to make it tho...

Embodiment 2

[0044] A method for tissue culture expansion and breeding of dendrobium comb lipped dendrobium comprises the following steps:

[0045] The steps of explant selection: select the strong female plant of Dendrobium comb lipped for 2 years; artificially pollinate the dendrobium dendrobium during the flowering period in late September every year, let it grow fruit pods, and after 3-4 months carefully Cultivate. When the pods are mature, pick the mature pods and store them in the refrigerator at 3-7°C for 2 weeks; when the pods are mature, the outer seed shell is yellow-green and intact.

[0046] Steps for disinfection of explants: Take out the mature fruit pods, soak them in detergent water or washing powder water for 15-20 minutes, brush the surface of the seed shells with a brush, and rinse them with tap water for 10 minutes; after rinsing, put them into a clean beaker, transfer Go to the inoculation room, soak in medical alcohol with a volume concentration of 75% for 30s in a st...

Embodiment 3

[0052] A method for tissue culture expansion and breeding of dendrobium comb lipped dendrobium comprises the following steps:

[0053] The steps of explant selection: select the strong female plant of Dendrobium spp. for 4 years; carry out artificial pollination to Dendrobium spp. during the flowering period in late September every year, let it grow fruit pods, and after 3-4 months carefully Cultivate. When the pods are mature, pick the mature pods and store them in the refrigerator at 3-7°C for 2 weeks; when the pods are mature, the outer seed shell is yellow-green and intact.

[0054] Steps for disinfection of explants: Take out the mature fruit pods, soak them in detergent water or washing powder water for 20 minutes, brush the surface of the seed shells with a brush, and rinse them with tap water for 10-15 minutes; after rinsing, put them into a clean beaker, transfer Go to the inoculation room, soak in medical alcohol with a volume concentration of 75% for 60s in a steril...

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Abstract

The invention discloses a tissue culture, propagation expanding and seedling growing method of dendrobium strongylanthum. The tissue culture, propagation expanding and seedling growing method of the dendrobium strongylanthum comprises the steps of selecting explants, disinfecting the explants, sowing, performing subculture, performing rooting culture, hardening seedling as well as domesticating and transplanting. By the tissue culture, propagation expanding and seedling growing method, the breeding speed and number of the dendrobium strongylanthum seedlings can be increased effectively, the stability of the germplasm is controlled from the source of a female parent, the quality of the seedlings is guaranteed, industrialized production of high-quality seeds and seedlings of the dendrobium strongylanthum is realized, the production requirement is met and the current situation of resource scarcity is relieved.

Description

technical field [0001] The invention relates to a tissue culture seedling raising technique, in particular to a method for tissue culture expansion and seedling cultivation of Dendrobium combifolia. Background technique [0002] At present, Dendrobium strongylanthum Rchb.f. is an orchid plant with erect, fleshy, cylindrical or long spindle-shaped stems and multiple nodes; racemes usually have 1-4, terminal or lateral On the upper part of the stem, the rachis of the inflorescence is slender, and more than 20 small flowers are densely grown. The flowers are yellow-green, the base of the sepals is purple-red, and the lips are purple-colored. The flowering period is September-October. It grows on the trunks of forests at an altitude of 1000-2100 meters or climbs on stones, mainly distributed in China's Hainan, south to west of Yunnan (Jinghong, Pu'er, Shuangjiang, Jingdong, Lvchun, Tengchong, Yingjiang), Myanmar , Thailand and other places. [0003] At present, the Dendrobium ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/00A01H4/008
Inventor 许冬瑾严新雷连海杜永魏
Owner 康美药业(昆明)种质资源有限公司
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