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Kit for determining content of sex hormone binding globulin and testing method of kit

A technology combining proteins and kits, applied in the field of biochemistry, can solve problems such as timely diagnosis of unwell emergency and clinical patients, cumbersome steps in radioimmunoassay, expensive reagents, etc., and achieves improved precision, shortened reaction time, and expanded detection range. Effect

Inactive Publication Date: 2018-06-29
TAIZHOU ZECEN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The steps of radioimmunoassay are cumbersome, the reagents are expensive, and supporting equipment is required and there is radioactive contamination
Enzyme-linked immunosorbent assay has long detection time, complicated operation, poor repeatability, and is not suitable for emergency and clinical patients in time for diagnosis.
Latex-enhanced immunoturbidimetric method is simple and fast to operate, but has low sensitivity and poor reproducibility at low values

Method used

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  • Kit for determining content of sex hormone binding globulin and testing method of kit

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Embodiment Construction

[0044] Preferred embodiments of the present invention are described below, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0045] The operation procedure of adopting the test kit test sample of the present invention is as follows:

[0046] 1. Sample collection

[0047] Use correct medical techniques to collect serum (severe hemolysis or lipemia samples cannot be used for determination), and the collected samples should not be kept at room temperature for more than 8 hours; if not tested within 8 hours, the samples should be placed in a refrigerator at 2-8°C ; If it needs to be stored or transported for more than 72 hours, it should be frozen at below -20°C and avoid repeated freezing and thawing. Return to room temperature before use, shake gently to mix.

[0048] 2. Preparation before experiment

[0049] ① Take a bottle of lotion a...

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Abstract

The invention discloses a kit for determining the content of sex hormone binding globulin (SHBG) in human serum by adopting magnetic particle chemiluminescence. The kit comprises a calibrator, a quality control material, a resistance agent, a magnetic particle agent and a luminescent substrate, wherein the resistance agent is prepared from fluorescein isothiocyanate labeled sex hormone-binding globulin coating antibody and an alkaline phosphatase labeled sex hormone-binding globulin labeling antibody; and the magnetic particle agent is a conjugate of magnetic particles and goat-anti FITC. By adopting the kit provided by the invention, a chemiluminescence technology and immunomagnetic particles are combined, a nearly homogenous reaction system is provided; in addition, a one-step reaction mode is adopted, the detection sensitivity and the precision are greatly improved, the detection range is enlarged, the reaction time is greatly shortened, is shorter than 25min from starting to add asample to obtaining a detection result and is obviously faster than the time of a similar kit; and in addition, multiple samples can be determined on a fully automatic chemiluminescence apparatus, sothat high-flux rapid determination of the sex hormone-binding globulin is realized, the accuracy is high, the specificity is strong, and the precision and the detection efficiency are greatly improved.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring the content of sex hormone binding protein (SHBG) in human body by using magnetic particle chemiluminescence method. Background technique [0002] Sex hormone binding protein (SHBG) is a glycoprotein responsible for the blood transport of testosterone and estradiol. SHBG is synthesized in the liver, and it binds with high affinity to testosterone and 5-dihydro-testosterone, and with slightly lower affinity to estradiol. Among the combined testosterone, 44%-60% are combined with SHBG; about 95% of estrogen is combined with specific SHBG in the blood circulation. [0003] The clinical symptoms show that in female hirsutism and masculinization, the SHBG content is only 50% of the normal value, while the free T content increases by almost 90%. Therefore, the determination of SHBG in such diseases can not only be used as a diagnostic index, but also can be u...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N21/76
CPCG01N33/6803G01N21/76G01N33/54326
Inventor 丁建华刘振世沈怡张誉严
Owner TAIZHOU ZECEN BIOTECH CO LTD
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