Comprehensive preparation method for seed oil, crude proteins and crude fibers of Suaeda salsa
The technology of Suaeda salsa and Suaeda salsa seeds is applied in the field of comprehensive preparation of Suaeda salsa seed oil and protein, and can solve the problems of low utilization rate, waste of resources, etc. rate effect
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example 1
[0029] A method for comprehensively preparing Suaeda salsa seed oil, crude protein and crude fiber, comprising the following steps:
[0030] (1) Ultrasonic enzymatic hydrolysis pretreatment: peel and pulverize the washed and dried Suaeda salsa seeds, add deionized water according to the ratio of solid to liquid 1:5, mix well, add compound enzyme a (made by cellulase , ligninase and helicase are mixed according to the ratio of 0.1:0.1:0.15), at a temperature of 40°C, with a power of 300w, ultrasonic extraction for 10min, and centrifugation to collect the emulsion, hydrolyzate and filter residue respectively;
[0031] (2) Supercritical extraction: take the filter residue of step (1), add entrainer absolute ethanol, at a temperature of 45° C., an extraction pressure of 25 MPa, extract with supercritical carbon dioxide for 1 hour, and centrifuge to collect emulsion, hydrolyzate and residue respectively;
[0032] (3) Secondary treatment of enzymatic hydrolysis: take the residue of ...
example 2
[0039] A method for comprehensively preparing Suaeda salsa seed oil, crude protein and crude fiber, comprising the following steps:
[0040] (1) Ultrasonic enzymatic hydrolysis pretreatment: peel and pulverize the washed and dried Suaeda salsa seeds, add deionized water according to the ratio of solid to liquid 1:10, mix well, add compound enzyme a (made by cellulase , ligninase and helicase are mixed according to the ratio of 0.2:0.17:0.2), at a temperature of 45°C, with a power of 360w, ultrasonic extraction for 15min, and centrifugation to collect the emulsion, hydrolyzate and filter residue respectively;
[0041] (2) Supercritical extraction: take the filter residue of step (1), add entrainer ethyl acetate, at a temperature of 48°C, an extraction pressure of 28MPa, extract with supercritical carbon dioxide for 1.2h, and centrifuge to collect emulsion, hydrolyzate and residue respectively;
[0042](3) Secondary treatment of enzymatic hydrolysis: take the residue of step (2)...
example 3
[0049] A method for comprehensively preparing Suaeda salsa seed oil, crude protein and crude fiber, comprising the following steps:
[0050] (1) Ultrasonic enzymatic hydrolysis pretreatment: peel and pulverize the washed and dried Suaeda salsa seeds, add deionized water according to the ratio of solid to liquid 1:12, mix well, add compound enzyme a (made by cellulase , ligninase and helicase are mixed according to the ratio of 0.4:0.25:0.3), at a temperature of 58.5°C, with a power of 462w, ultrasonic extraction for 25min, and centrifugation to collect the emulsion, hydrolyzate and filter residue respectively;
[0051] (2) Supercritical extraction: take the filter residue of step (1), add entrainer ethyl acetate, at a temperature of 60°C, extraction pressure of 32.5MPa, supercritical carbon dioxide extraction for 1.7h, centrifuge to collect emulsion, hydrolyzate and residue respectively ;
[0052] (3) Secondary treatment of enzymatic hydrolysis: take the residue of step (2), ...
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