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A cobalt porphyrin/porous silica composite particle with photoacoustic imaging performance and cell protection function, and preparation method and application thereof

A technology of porous silica and composite particles, which is applied in the fields of biomedicine and nanomaterials, and can solve problems such as complex synthesis methods, poor degradability, and obstacles to the clinical application of inorganic nanostructures

Active Publication Date: 2020-10-23
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been related studies using Prussian blue nanoparticles, nano-gold rods, etc. for photoacoustic imaging (references: [1] KimT, Lemaster JE, Chen F, Li J, Jokerst JV. Blue-Poly(L-lysine) Nanocomplexes. Acs Nano. 2017; 11:9022-32. [2] Jesse V. Jokerst, Mridhula Thangaraj, Paul J. Kempen, Robert Sinclair, and Sanjiv S. Gambhi, Photoacoustic Imaging of Mesenchymal Stem Cells in Living Mice via Silica-Coated GoldNanorods.Acs Nano.2012; 6:5920–5930.), but the synthesis methods of these materials are complex, the degradability is poor, and it is difficult to maintain long-term stable biological safety, which hinders the development of these inorganic nanomaterials. Clinical Applications of Structures

Method used

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  • A cobalt porphyrin/porous silica composite particle with photoacoustic imaging performance and cell protection function, and preparation method and application thereof
  • A cobalt porphyrin/porous silica composite particle with photoacoustic imaging performance and cell protection function, and preparation method and application thereof
  • A cobalt porphyrin/porous silica composite particle with photoacoustic imaging performance and cell protection function, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of Porous Silica Composite Particles Carrying Cobalt Porphyrin

[0036] Disperse 40mg of porous silica nanoparticles in 20mL of ethanol solution, sonicate for 20min at an ultrasonic power of 40-60KHz, add 40mg of CoPP to it, and stir for 24h to make CoPP fully adsorbed inside the porous silica particles, and the reacted solution Centrifuge at 30,000 rpm, wash twice with deionized water, collect and dry for later use, and the prepared CoPP has a loading rate of about 2.5%.

[0037] figure 1 High-resolution transmission electron microscope and scanning electron microscope photos of the porous silicon oxide composite particles loaded with cobalt porphyrin prepared in this example. Scanning electron microscopy of the material showed a particle size of approximately 500 nm.

[0038] figure 2 The comparative area of ​​the cobalt porphyrin / porous silica composite particles prepared for this example is about 631.3m 2 / g (A) and average pore size 2.6nm (...

Embodiment 2

[0040] Example 2 Preparation of Porous Silica Composite Particles Carrying Cobalt Porphyrin

[0041] Disperse 40mg of porous silica nanoparticles in 30mL of ethanol solution, ultrasonically treat for 30min, and the ultrasonic power is 40-60KHz, add 50mg of CoPP to it, and stir for 24h, so that CoPP is fully adsorbed inside the porous silica particles, and the solution after reaction Centrifuge at 30000rpm, wash twice with deionized water, collect and dry for later use.

Embodiment 3

[0042] Example 3 Preparation of Porous Silica Composite Particles Carrying Cobalt Porphyrin

[0043] Disperse 40mg of porous silica nanoparticles in 40mL of ethanol solution, ultrasonically treat for 40min, and the ultrasonic power is 40-60KHz, add 60mg of CoPP to it, and stir for 24h, so that CoPP is fully adsorbed inside the porous silica particles, and the solution after reaction Centrifuge at 30000rpm, wash twice with deionized water, collect and dry for later use.

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Abstract

The invention relates to cobalt porphyrin / porous silicon dioxide composite particle with photoacoustic imaging performance and a cytoprotection function and a preparation method and application thereof. The preparation method comprises the steps that a cobalt ion-centered metallic porphyrin compound and porous silicon dioxide nano particles are mixed and stirred, particles are centrifugally collected and dried, and the multifunctional cobalt porphyrin / porous silicon dioxide composite particle is obtained. According to the preparation method, the metallic porphyrin compound is carried by the porous silicon dioxide nano particles, an optical absorption property of a porphyryl compound is changed, the absorption of molecule near an infrared light area is promoted, and an expressed photoacoustic imaging radiography performance is good. According to the composite material, haem oxygenase-1 can be up-regulated to generate a cytoprotection effect, and the survival rate of a cell after cell transplantation is improved. The adopted preparation method is simple, quick, economical, applicable and has high repeatability, the photoacoustic imaging and the cytoprotection are successfully combined, a new method for an imaging mark is provided and improves the cell survival rate.

Description

technical field [0001] The invention relates to the technical fields of biomedicine and nanometer materials, in particular, a cobalt porphyrin / porous silicon oxide composite particle with photoacoustic imaging performance and cell protection function, its preparation method and application. Background technique [0002] Mesenchymal stem cells are a type of stem cells with multiple differentiation potentials. Under specific conditions, they can differentiate into specific types of cells. They are considered to be an important source for repairing damaged tissues and organ transplantation. However, stem cell therapy is currently limited by the low survival rate of stem cells after transplantation, and it is difficult to monitor the survival efficiency of stem cells dynamically and in real time, so it cannot be truly applied clinically. [0003] Metalloporphyrins (MPs) are a large family of organic compounds with a variety of metabolic functions, and their biochemical functions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/22
CPCA61K49/225
Inventor 姚明华吴蓉
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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