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Pakchoi free microspore induction medium formula

A technology for inducing culture medium and microspores, which is applied in the field of free microspore induction medium formulation of pakchoi, can solve the problems of low induction rate of microspore embryogenic callus, slow research progress and instability, and achieves high industrial promotion value. , the effect of improving the induction efficiency

Inactive Publication Date: 2018-06-22
张瑞明
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, the research on the cultivation of free microspores of pakchoi has been slow, and there are few reports on the use of this technology for the breeding of pakchoi. Application of Spore Culture Technology in Breeding

Method used

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  • Pakchoi free microspore induction medium formula

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Embodiment

[0019] Prepare a kind of pakchoi free microspore induction medium of the present invention, and the medium formula is specific as follows:

[0020] KNO 3 60mg / L, MgSO 4 ∙7H 2 O 62.5mg / L, KH 2 PO 4 67.5mg / L, Ca(NO 3 ) 2 ∙4H 2 O 225mg / L, Na 2 -EDTA 12.5mg / L, FeSO 4 ∙7H 2 O 13.5mg / L, MnSO 4 ∙4H 2 O 25mg / L, NiNO 3 80mg / L,H 3 BO 3 8.25mg / L, ZnSO 4 ∙7H 2 O 9mg / L, KI 0.425mg / L, Na 2 MoO 4 ∙2H 2 O 0.25mg / L, CuSO 4 ∙5H 2 O 0.025mg / L, CoCl 2 ∙6H 2 O 0.025mg / L, RNA-Na 0.25g / L, 2-hydroxyethyltrimethylammonium chloride 140mg / L, L-glutamine 500mg / L, vitamin B1 0.5mg / L, vitamin B6 0.5mg / L, Niacin 5mg / L, folic acid 0.05mg / L, biotin 0.05mg / L, inositol 160mg / L, glycine 4mg / L, L-glutathione 30mg / L, methylnitrosourea 1.5g / L , sucrose 130g / L, plant gel 3.25g / L, 6-BA 0.2mg / L, NAA 0.35mg / L, polyethylene glycol 0.16g / L, hydrolyzed protein 1.5g / L, activated carbon 0.02g / L, Phytosulfokinetin PSK-α 45pmol / L, pH 5.4.

[0021] In the autumn of 2013, the seeds of the pakchoi v...

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Abstract

The invention provides a pakchoi free microspore induction medium. The formula comprises KNO3, MgSO4 7H2O, KH2PO4, Ca(NO3)2 4H2O, Na2-EDTA, FeSO4 7H2O, MnSO4 4H2O, NiNO3, H3BO3, ZnSO4 7H2O, KI, Na2MoO4 2H2O, CuSO4 5H2O, CoCl2 6H2O, RNA-Na, choline hydrochloride, L-glutamine, vitamin B1, vitamin B6, niacin, folic acid, biotin, inositol, glycine, L-glutathione, methylnitrosourea, sucrose, phytagel,6-BA, NAA, polyethylene glycol, hydrolyzed protein, activated carbon and phytosulfokine PSK-alpha. The medium can greatly improve the induction efficiency of embryogenic calluses of free microspores of pakchoi.

Description

technical field [0001] The invention relates to a pakchoi free microspore induction medium formula, in particular to a pakchoi free microspore induction medium formula that can greatly improve the induction efficiency of pakchoi free microspore embryogenic callus and has important application value. It belongs to the high-tech field of vegetable cultivation. Background technique [0002] Chinese cabbage ( Brassica campestris L. ssp. chinensis ) belongs to Brassica Brassica Brassica subspecies of Brassica cabbage, also known as non-heading cabbage, green cabbage, small rapeseed, including Chinese cabbage (common cabbage), Chinese cabbage, Chinese cabbage, Chinese cabbage and other varieties, native to my country is a vegetable crop widely cultivated in various parts of our country. Because of its strong edibility, rich nutrition, high quality, and various flavors, it is deeply loved by people. Moreover, pakchoi has a short production period, strong adaptability, and low p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张瑞明
Owner 张瑞明
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