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Hexuronate C4-epimerase mutant with improved conversion activity, and method for producing D-tagatose by using same

A technology of epimerase and hexuronate, applied in the direction of racemase/epimerase, biochemical equipment and methods, isomerase, etc., can solve the problem of difficult supply of raw milk and low price Stability and other issues

Active Publication Date: 2018-06-08
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price of lactose as a raw material for tagatose is unstable depending on the production volume, the supply and demand of raw milk and lactose in the global market, etc., and such price fluctuations in raw milk make it difficult to produce a stable supply of raw materials for tagatose

Method used

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  • Hexuronate C4-epimerase mutant with improved conversion activity, and method for producing D-tagatose by using same
  • Hexuronate C4-epimerase mutant with improved conversion activity, and method for producing D-tagatose by using same
  • Hexuronate C4-epimerase mutant with improved conversion activity, and method for producing D-tagatose by using same

Examples

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Effect test

example 1

[0092] Example 1 Design and Analysis of Improved Target Sites

[0093] Orthologs (orthologs) expected to have homology to the amino acid sequence of the hexuronate C4-epimerase (hereinafter referred to as wild type) derived from Thermotoga neapolitana , homologous genes expected to have the same function in other microbial species) analysis of the tertiary model structure of the active site, mainly selecting amino acids expected to be functionally important. Design of improved target sites to enhance D-fructose based on analysis of docking models between structures refined after alanine-scanning mutagenesis analysis for selected amino acids and D-fructose The C4-epimer unit is active. Specific embodiments will be explained below.

example 1-1

[0094] The analysis of example 1-1 ortholog (ortholog)

[0095] The ortholog (ortholog) with homology to the wild-type amino acid sequence (SEQ ID NO: 1) was selected from the GenBank database (about 60 sequence coverage (sequence coverage) was 80% or higher and homologous Homology is 50% or greater ortholog). Conserved amino acids expected to be functionally important were identified by multiple sequence alignment analysis of selected orthologs.

example 1-2

[0096] The analysis of the tertiary structure form of example 1-2 enzyme

[0097] Because the Protein Data Bank (Protein Data Bank) shows no protein structure with 30% or higher amino acid sequence identity (Identity) with wild-type and orthologs, it is expected that the model structure of wild-type predicted by homology modeling may be Inaccurate. Therefore, the active site structure between the models obtained by using various algorithms through various structural modeling servers (RaptorX, Robetta, ModWeb, M4T, HHpred, PHYRE2, I-TASSER, SWISS-MODEL, etc.) was compared, thereby Information on commonly expected structural sites is obtained and used in the next step.

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Abstract

The present invention relates to a hexuronate C4-epimerase mutant having improved conversion activity of hexuronate C4-epimerase, and a method for producing tagatose using the same.

Description

technical field [0001] The present invention relates to a hexuronate C4-epimerase mutant having improved conversion activity, and a method for producing D-tagatose using the same. Background technique [0002] Tagatose is a natural sweetener that exists only in small amounts in foods such as milk, cheese, cocoa, etc., and naturally sweet fruits such as apples and mandarin oranges, and has similar physical properties to sucrose. Tagatose has a low calorie value of 1.5 kcal / g, equivalent to one-third of the calorie value of sucrose, and a glycemic index (glycemic index, GI) of 3, equivalent to 5% of the glycemic index of sucrose. Tagatose tastes sweet like sucrose and has various health and functional characteristics, and thus has been used in a variety of products as an alternative sweetener capable of satisfying taste and health. [0003] Tagatose can be produced from galactose as a raw material by a chemical isomerization (catalytic reaction) method and a biological (enzym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/90C12N1/20C12P19/02C12R1/19
CPCC12N9/90C12Y501/02C12N1/20C12P19/02C12N1/205C12R2001/19C12P19/24
Inventor 梁成才金良姬朴逸香李英美赵显国金成俌朴承源
Owner CJ CHEILJEDANG CORP
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