Identification method for anoectochilus roxburghii with different cultivation methods

A calculation method and technology of tissue culture seedlings are applied in the directions of measuring devices, material analysis by optical means, instruments, etc., which can solve the problems of insignificant differences, chaotic sales market, hindering the healthy development and scale expansion of the clematis industry, etc. Achieving good application prospects

Inactive Publication Date: 2018-06-08
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The identification of clematis in different cultivation methods on the market is limited to empirical judgment, but the difference between 3-month-old bottle seedlings, 4-month-old bottle seedlings, or 6-month-old seedlings planted in the soil is not significant, leading some unscrupulous merchants to directly sell Tissue-cultured bottle seedlings were put on the market, shoddy as good ones, causing confusion in the sales market, and seriously hindering the healthy development and scale expansion of the clematis industry

Method used

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  • Identification method for anoectochilus roxburghii with different cultivation methods
  • Identification method for anoectochilus roxburghii with different cultivation methods
  • Identification method for anoectochilus roxburghii with different cultivation methods

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Experimental program
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Embodiment 1

[0028] Embodiment 1 Detection method of the present invention

[0029] (1) Sample treatment: wash the fresh samples to be tested in batches for 3 to 5 times until the water becomes clear and there is no sediment on the surface of the samples, place them in a 60°C oven to dry to constant weight, crush them through a 60-mesh sieve, and take an appropriate amount for each batch. Put it into the finger tube and tamp it so that the sample height is between 2cm, to be tested;

[0030] (2) Spectrum acquisition and preprocessing: Scan the sample with NIRFlex N-500 near-infrared spectrum analyzer, acquisition parameters: scanning range is 5000-10000cm -1 , the number of scans is 32, and the resolution is 8cm -1 , each sample is reloaded and scanned 3 times. Before each scan, the sample in the finger tube should be tamped to make the sample even and flat. The system will automatically store the NIRS spectrum of the scanned sample into the spectral database; in the NIRCal 5.4 data proce...

Embodiment 2

[0036] Embodiment 2 detection method of the present invention

[0037] (1) Sample treatment: wash the fresh samples to be tested in batches for 3 to 5 times until the water becomes clear and the surface of the samples is free of sediment, put them in a 55°C oven and dry them to constant weight, crush them through a 100-mesh sieve, and take an appropriate amount for each batch. Put it into the vial and tamp it down so that the height of the sample is between 1cm, to be tested;

[0038] (2) Spectrum acquisition and preprocessing: Scan the sample with NIRFlex N-500 near-infrared spectrum analyzer, acquisition parameters: scanning range is 5000-10000cm -1 , the number of scans is 32, and the resolution is 8cm -1 , each sample is reloaded and scanned 3 times. Before each scan, the sample in the finger tube should be tamped to make the sample even and flat. The system will automatically store the NIRS spectrum of the scanned sample into the spectral database; in the NIRCal 5.4 data...

experiment example 1

[0046] Experimental Example 1 Identification of different cultivation methods of Auranthus clematis

[0047] 1 Experimental materials

[0048] 1.1 Instrument

[0049] NIRFlex N-500 near-infrared spectrometer, solid measuring cell, accessories for 6-position vial, sample rotator diffuse reflection integrating sphere, NIRCal 5.4 software for data processing and modeling (Buchi Labortechnik AG company).

[0050] DF-15 desktop continuous feeding pulverizer (Wenling Linda Machinery Co., Ltd.), 60-mesh standard inspection sieve (60-mesh aperture, 0.3mm, Shangyu Yinhe Test Instrument Factory), DHG-9030H constant temperature oven (Shanghai Jinghong Experimental Equipment Ltd.).

[0051] 1.2 Experimental samples

[0052] Collect 30 batches of clematis in different cultivation methods, including 10 batches of 3-month-old bottle seedlings, 4-month-old bottle seedlings, and 6-month-old clematis planted in the soil; the samples come from Nanjing Geyuan Agriculture and Forestry Technolog...

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Abstract

The invention discloses an identification method for anoectochilus roxburghii with different cultivation methods; a near infrared spectroscopy analysis technology is adopted for identification, wherein the method includes the following steps: (1) taking a to-be-detected sample powder, loading the sample, and collecting a near infrared spectrogram; and (2) adopting a PCA-Cluster clustering analysismethod as a calculation method, selecting unit length normalization (nle) and first-order smooth derivation (dgl) pretreatment for pretreating the near infrared spectrogram, and identifying the anoectochilus roxburghii with different cultivation methods by score values of sample main components. The identification method has the advantages of being simple, fast, accurate, green and environmentally friendly, has no damage to the sample, can effectively identify 3-month bottle seedlings, 4-month bottle seedlings or 6-month in-soil planted seedlings of anoectochilus roxburghii, and has good application prospect.

Description

technical field [0001] The invention relates to a method for identifying clematis in different cultivation modes. Background technique [0002] Anoectochilus roxburghii is a plant of the genus Orchidaceae, mainly distributed in Fujian and Taiwan regions of my country, and is a rare and endangered perennial medicinal material. Auranthus clematis has the effect of clearing away heat and cooling blood, dispelling wind and dampness, and it is mainly used to treat diseases such as nephritis, cystitis, diabetes, bronchitis, and convulsions in children. Clematis clematis has high medicinal and health care value, but wild resources are scarce, and currently the market is dominated by cultivated products. The cultivation methods are mainly divided into tissue culture and planting in the soil. Most of the tissue culture products are tissue culture bottle seedlings of 3 to 5 months old, and most of the soil planting products are 3 to 5 month old bottle seedlings transplanted into the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3563G01N21/359
CPCG01N21/3563G01N21/359G01N2021/3572
Inventor 张勋林羽徐伟陈抒云许文黄泽豪
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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