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A kind of perfluorocarbon albumin nanoparticles and preparation method and application thereof

A technology of albumin nanoparticles and perfluorocarbons, which is applied in the field of preparation of albumin nanoparticles, can solve problems such as high requirements, affecting drug safety, and limiting the application of albumin nanoparticles-related preparations, so as to improve biological safety, The preparation process is simple and easy, and the effect of low equipment requirements

Active Publication Date: 2020-12-22
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cross-linking method needs to introduce a highly toxic cross-linking agent, which seriously affects the safety of medication
The high-pressure homogenization method has extremely high requirements on equipment and the prepared albumin nanoparticles have weak loading capacity
These deficiencies limit the clinical application of albumin nanoparticle-related preparations

Method used

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  • A kind of perfluorocarbon albumin nanoparticles and preparation method and application thereof
  • A kind of perfluorocarbon albumin nanoparticles and preparation method and application thereof
  • A kind of perfluorocarbon albumin nanoparticles and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041]Preparation of perfluorotributylamine (PFTBA)-albumin nanoparticles:

[0042]Measure 0.5 mL of human serum albumin stock solution (10 g / 50 mL) into a 7 mL vial, add deionized water to dilute to 2 mL, and mix at 20°C. Then add 1.5 mL of perfluorotributylamine (PFTBA), then add 1.0 mL of ethanol (analytical grade), and mix well. Use an ultrasound probe to penetrate into the vial to near the bottom of the liquid, use 500W power during ultrasound, set the ultrasound to stop 3 seconds after every 2s, ultrasound 3 times, 2 minutes each time, the preparation needs to be placed in a water bath at 20℃ after each ultrasound 1-2 minutes. The sample was transferred to a 4 mL centrifuge tube, centrifuged at 3000 rpm for 3 min, and the supernatant was taken to obtain the prepared perfluorotributylamine albumin nanoparticles.

[0043]The morphology of perfluorotributylamine-albumin nanoparticles was observed by transmission electron microscope (TEM). First, the prepared preparation is diluted 160 ...

Embodiment 2

[0045]Preparation of perfluorooctyl bromide (PFOB)-albumin nanoparticles:

[0046]Measure the stock solution of human serum albumin and add water to dilute to an albumin concentration of 5g / 100mL, and mix at 25°C. Take 2 mL of the above diluted solution, add 3 g of perfluorooctyl bromide (PFOB), then add 1.0 mL of ethanol (analytical grade), and mix well. Use the ultrasonic probe to penetrate deep into the bottle to near the bottom of the liquid for ultrasonic treatment. Transfer the sample to a centrifuge tube, centrifuge at 3000 rpm for 3 minutes, and take the supernatant, which is the prepared perfluorooctyl bromoalbumin nanoparticles with an average particle size of 220 nm.

Embodiment 3

[0048]Preparation of perfluorotripropylamine (PFTPA) + perfluorodecalin (FDC)-albumin nanoparticles:

[0049]Measure the stock solution of human serum albumin and add water to dilute to an albumin concentration of 5g / 100mL, and mix at 30°C. Take 2 mL of the above diluted solution, add 0.33 g of perfluorotripropylamine and 0.79 g of perfluorodecalin, then add 1.0 mL of ethanol (analytical purity), and mix well. Use the ultrasonic probe to penetrate deep into the bottle to near the bottom of the liquid for ultrasonic treatment. Transfer the sample to a centrifuge tube, centrifuge at 3000 rpm for 3 minutes, and take the supernatant, which is the prepared perfluorotripropylamine perfluorodecalin albumin nanoparticles, with an average particle size of 150nm.

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Abstract

The invention discloses perfluorocarbon albumin nanoparticles which comprise albumins and perfluorocarbon compounds, wherein the albumins coat the perfluorocarbon compounds, and the mass ratio of theperfluorocarbon compounds to the albumins is (4-40): 1. The perfluorocarbon albumin nanoparticles have good biological safety as a novel blood platelet inhibitor. The perfluorocarbon albumin nanoparticles which are administrated will induce great reduction of quantity of blood platelets in a human body, various functions of the blood platelets can be also inhibited obviously, and the perfluorocarbon albumin nanoparticles have relatively good clinical applications and practical treatment meaning.

Description

Technical field[0001]The invention belongs to the field of biomedicine, and specifically relates to a preparation method of albumin nanoparticles based on platelet function inhibition.Background technique[0002]Platelets are small pieces of cytoplasm with biological activity, which are derived from the lysis and shedding of mature megakaryocyte cytoplasm, and are of great significance to the realization of physiological and pathological processes such as hemostasis, thrombosis, inflammation, and wound healing. The process of platelet adhesion between platelets is platelet aggregation, which participates in physiological or pathological hemostasis, and it is a process of blood coagulation. Thrombocytosis can cause abnormalities in the body, and thrombocytosis is often secondary to polycythemia, chronic myeloid leukemia, acute bleeding or hemolysis, malignant tumors, bone marrow fibrosis, and after splenectomy.[0003]Clinical investigations have shown that the occurrence and development...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/38A61K9/50A61K47/06A61P7/02A61P19/08A61P35/00
CPCA61K9/5015A61K38/385
Inventor 胡一桥吴锦慧黄晴张宝丽
Owner NANJING UNIV
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