DNA barcode primers, DNA barcodes, kits, methods and applications for rapid identification of Arthrospora adenophagous strains
A technology of Arthropodium adenosine and barcode primers, applied in the direction of DNA/RNA fragments, recombinant DNA technology, and methods based on microorganisms, can solve the problems of damaging the economic interests of Pu'er tea production enterprises and the lack of Pu'er tea, so as to ensure health Prosperous development, improved identification accuracy, and easy comparison
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[0074] Example 1 Based on the homology comparison of the sequences amplified with the first, second, third and fourth primer pairs to determine whether the strain to be tested is Arthrospora adenophagous TMCC 70007
[0075] 1. Source of strains: See Table 1 below for information on the selected strains:
[0076] Table 1
[0077]
[0078] In the above table 1, the TMCC 70007 strain was preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number CGMCCNo.8683; the CBS strains were all purchased from the CBS-KNAW Fungal Biodiversity Center, the Netherlands .
[0079] 2. Extract the strain DNA separately: OMEGA E.Z.N.A. TM Yeast DNA Kit D3370-01 (manufactured by OMEGA) was used to extract yeast genomic DNA, and the DNA concentration of the sample was diluted to 0.5 μg / μL with sterilized deionized water.
[0080] 3. To amplify the DNA fragments, respectively adopt the following 4 pairs of primer pairs to carry out the polymera...
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