Construction method and application of animal model suffering from fetal-origin adult osteoporosis
An osteoporosis and animal model technology, applied in medical preparations containing active ingredients, organic active ingredients, pharmaceutical formulations, etc., can solve the problems of slow progress in laboratory research and lack of model establishment of fetal-derived osteoporosis. , to achieve the effect of improving the quality of the population
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[0029] The features and advantages of the present invention can be further understood through the following detailed description in conjunction with the accompanying drawings. The examples provided are only illustrative of the method of the present invention and do not limit the rest of the present disclosure in any way.
[0030] [Example 1] Occurrence of osteoporosis in offspring rats as adults caused by caffeine exposure during pregnancy
[0031] 1. Experimental animals
[0032] SPF grade healthy Wistar rats were purchased from Hubei Provincial Center for Disease Control and Prevention, animal license number: SCXK (E) 2008-2010. This study was approved by the Ethics Committee of Wuhan University Medical Center, and was carried out in strict accordance with the relevant treatment guidelines of international laboratory animal protection certification and evaluation institutions.
[0033] The experimental animals were kept in a barrier environment with a temperature of 22-25°...
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