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Detection method and detection kit for goat lmcd1 gene single nucleotide polymorphism site

A technology of single nucleotide polymorphism and polymorphic sites, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve the problems of unreported correlation of polymorphic growth traits, etc., and achieve low cost , high precision effect

Inactive Publication Date: 2018-08-17
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

The research on the polymorphism of goat LMCD1 gene and its relationship with growth traits has not been reported so far

Method used

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  • Detection method and detection kit for goat lmcd1 gene single nucleotide polymorphism site
  • Detection method and detection kit for goat lmcd1 gene single nucleotide polymorphism site
  • Detection method and detection kit for goat lmcd1 gene single nucleotide polymorphism site

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0036] In the present invention, firstly, primers are designed according to the conserved sequence of the LMCD1 gene, and the genomic DNAs of four goat populations are respectively used as templates to carry out PCR amplification, and the PCR products are mixed, purified, and sequenced. Then, carry out sequencing map analysis and sequence comparison to screen out SNP sites; secondly, carry out PCR-SSCP detection of polymorphic sites in the tested population; finally, carry out statistical analysis of population genetics according to the genotypes detected in the population The association analysis with body size traits screened out molecular markers closely related to goat body size traits. The present invention will be described in detail below, which is an explanation of the present invention rather than a limitation.

[0037] (1), Amplification of G...

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Abstract

The invention discloses a detection method and detection kit for goat LMCD1 gene mononucleotide polymorphism sites. The method comprises the following steps: by using to-be-detected goat genome DNA (deoxyribonucleic acid) of containing LMCD1 gene as a template, carrying out PCR (polymerase chain reaction) amplification on the goat LMCD1 gene, and carrying out SSCP (single strand conformation polymorphism) polymorphism detection. The gene polymorphism sites comprise the following base polymorphisms: the 681st site of the goat LMCD1 gene is A or G; and the 2825th site of the goat LMCD1 gene is C or T. The invention provides a simple quick low-cost high-precision detection method, which can be conveniently applied to screening and detecting of genetic markers closely related to goat growth traits at the DNA level and can be used for assisted selection and molecular breeding of goat.

Description

technical field [0001] The invention relates to the detection of gene single nucleotide polymorphism (SNP), in particular to the detection of goat LMCD1 gene single nucleotide polymorphism site. Background technique [0002] Single nucleotide polymorphism (SNP) refers to the polymorphism caused by the substitution of a single nucleotide (A / T / C / G) in the genomic DNA sequence, mainly caused by the conversion or transversion of a single base . SNPs with transformational variants accounted for about 2 / 3, and several other SNPs were at similar levels. The cytosine of the CpG dinucleotide is the most mutated site in the genome, most of which are methylated and can be spontaneously deaminated to form thymine. [0003] According to the influence on genetic traits, gene polymorphism can be divided into two types: one is synonymous mutation polymorphism, that is, the change of coding sequence caused by SNP does not affect the amino acid sequence in the protein translated by it, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858
CPCC12Q1/686C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/158
Inventor 陈宏房兴堂刘宣宣张春雷金晶王艳红
Owner XUZHOU NORMAL UNIVERSITY
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